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Updated: Feb 3, 2026

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

Published on: July 15, 2018

新鮮凍結腎細胞癌検体（UroCCR 9）におけるRNA完全性に関する危険因子

G Margue¹, G Capon², B C Niort³

¹Department of Urology - CHU Bordeaux, Bordeaux, France; I.CaRe Bordeaux - BRIC INSERM U1312, Bordeaux, France; Kidney Cancer group of the Oncology Committee of the French Association of Urology, Paris, France.

The French journal of urology

|February 1, 2026

要旨

Introduction:

High-quality biospecimens are essential for transcriptomic analyses in renal cell carcinoma (RCC), yet real-world determinants of RNA integrity in routine surgical settings remain insufficiently documented. This study aimed to evaluate RNA quantity and quality from cryopreserved RCC tissues and identify factors associated with RNA degradation.

Material And Methods:

We conducted a retrospective analysis of 160 patients with clear cell RCC included in the UroCCR network (2005-2012). Tumour and matched normal kidney tissues were prospectively collected during partial or radical nephrectomy and snap-frozen. RNA was extracted using an automated protocol. RNA concentration was measured by NanoDrop spectrophotometry, and RNA quality was assessed using the RNA Quality Score (RQS). Multivariate linear regression models with random intercepts were used.

Results:

RNA extraction was successful in 302 of 310 samples. RNA concentration exceeded 25 ng/µL in 93% of samples, and 78.4% met both quantitative and qualitative criteria (RQS ≥ 6). Mean RNA concentration was 128.8 ± 104.4 ng/µL, and mean RQS was 7.52 ± 1.40. Tumour tissue showed higher RNA quality than matched healthy tissue (mean RQS: 8.33 ± 1.02 vs 6.66 ± 1.30; p < 0.001). Preoperative embolization was independently associated with lower RNA quality (p < 0.001). Freezing delay (mean 30 ± 25 min) and duration of storage were not associated with RNA degradation.

Conclusion:

High-quality RNA can be extracted from fresh-frozen RCC tissues under routine clinical conditions. RNA integrity is mainly influenced by tumour biology rather than short procedural delays, supporting biologically informed biobanking strategies.

キーワード:

RNA品質腎細胞癌バイオバンキング凍結保存

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