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Updated: Feb 4, 2026

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Published on: January 2, 2018

シス型かトランス型か：パーキン活性化メカニズムの謎

Mohini Sherawat¹, Ankit Kumar¹, Dipti Ranjan Lenka¹

¹Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Bhopal, Bhopal, 462066, India.

Essays in biochemistry

|February 2, 2026

Abstract:

The PARK2 gene, which encodes the E3 ubiquitin ligase Parkin, and the PARK6 gene, encoding phosphatase and tensin homolog (PTEN)-induced kinase 1 (PINK1), are frequently mutated in patients with Parkinson's disease (PD). Parkin is normally maintained in an autoinhibited conformation, and its activation is triggered by PINK1-mediated phosphorylation of both ubiquitin or NEDD8 and Parkin's ubiquitin-like (Ubl) domain. This review provides a comprehensive overview of the models proposed over the past decade to explain Parkin's autoinhibition and activation. We summarize key structural and biophysical studies that have progressively uncovered the molecular basis of Parkin activation, tracing the evolution of these insights. This review concludes by discussing the intriguing and still unresolved question of whether Parkin activation occurs through a cis or trans mechanism and outlines future directions for research aimed at understanding these pathways.

キーワード:

PINK1 パーキンパーキンソン病ミトファジーユビキチン

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The Use of the Puzzle Box as a Means of Assessing the Efficacy of Environmental Enrichment

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Published on: December 29, 2014

Quantitative Comparison of cis-Regulatory Element CRE Activities in Transgenic Drosophila melanogaster

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