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Updated: Feb 4, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

キネトプラスト類およびジプロネマ類のミトコンドリアゲノムの組み立てとアノテーション

Evgeny S Gerasimov^1,2, Matus Valach³, Gertraud Burger³

¹Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.

Methods in molecular biology (Clifton, N.J.)

|February 2, 2026

Abstract:

This chapter outlines the assembly and annotation of mitochondrial genomes and transcriptomes of kinetoplastids and diplonemids using high-throughput sequencing data. The mitochondrial genomes of these protists are highly atypical, characterized by multipartition and extensive RNA editing. These complexities pose significant challenges for genome assembly, gene prediction, and transcript reconstruction. Here, we describe analytical strategies developed in our laboratories that address these issues.

キーワード:

ジプロネマ類キネトプラストミトコンドリオン次世代シーケンシング RNA編集トリパノソーマ類

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Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

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