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CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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RNA Editing

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...

Genomics

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...

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Genomic Imprinting and Inheritance

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Updated: Feb 4, 2026

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

ゲノム編集を用いたユーグレナ・グラシリスにおけるワックスエステル組成の工学的改変

Sakura Nagamine¹, Rikuto Oishi¹, Masami Nakazawa²

¹Department of Applied Biochemistry, Faculty of Agriculture, Osaka Metropolitan University, Sakai, Osaka, Japan.

Methods in molecular biology (Clifton, N.J.)

|February 2, 2026

まとめ

この要約は機械生成です。

本研究では、ワックスエステル生産を改変するためのユーグレナ・グラシリスのクリスパー/キャス9ゲノム編集プロトコルを詳述する。この方法により、合成生物学およびバイオ製造アプリケーションのためにユーグレナ・グラシリスを安定的に遺伝子改変することが可能になる。

キーワード:

嫌気的代謝 CRISPR/Cas9 ユーグレナ・グラシリス脂肪酸β酸化ゲノム編集機能喪失代謝改変標的遺伝子ノックアウトワックスエステル合成

さらに関連する動画

Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

関連する実験動画

Last Updated: Feb 4, 2026

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

科学分野:

合成生物学
代謝工学
ゲノミクス

背景:

ゲノム編集の進歩により、ユーグレナ・グラシリスのような非モデル生物における代謝工学が可能になった。
CRISPR/Cas9およびCRISPR/Cas12aシステムがユーグレナ・グラシリスのゲノム編集のために確立されている。

研究の目的:

ユーグレナ・グラシリスにおけるCRISPR/Cas9ベースのゲノム編集の詳細なプロトコルを提供すること。
嫌気条件下でのワックスエステル組成の安定した改変を可能にすること。

主な方法:

逆β酸化経路の主要酵素を標的とするためのCRISPR/Cas9技術の利用。
ユーグレナ・グラシリスにおけるワックスエステル鎖長を変化させるためのノックアウト変異体の生成。

主要な成果:

ワックスエステの鎖長が変化したユーグレナ・グラシリスのノックアウト変異体を生成した。
ユーグレナ・グラシリスの代謝の再現可能で安定した遺伝子改変方法を確立した。

結論:

開発されたCRISPR/Cas9プロトコルは、ユーグレナ・グラシリスの代謝の安定した遺伝子改変を促進する。
このアプローチは、合成生物学およびバイオ製造のためのグリーンシャーシとしてのユーグレナ・グラシリスの使用をサポートし、ノックイン戦略の可能性を秘めている。