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関連する概念動画

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Phase II Reactions: Methylation Reactions01:17

Phase II Reactions: Methylation Reactions

730
Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...
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DNA Topoisomerases02:02

DNA Topoisomerases

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Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
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DNA Helicases00:55

DNA Helicases

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DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
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Recombinant DNA01:09

Recombinant DNA

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Overview
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DNA Replication02:40

DNA Replication

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DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
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関連する実験動画

Updated: Feb 4, 2026

Laser Capture Microdissection of Mammalian Tissue
16:34

Laser Capture Microdissection of Mammalian Tissue

Published on: October 1, 2007

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レーザーキャプチャーマイクロダイセクションとそれに続くDNAメチル化プロファイリング

Lu Liu1, Yingchuo Hu2,3,4, Chunhong Zheng5

  • 1Changping Laboratory, Beijing, China. luliu@cpl.ac.cn.

Methods in molecular biology (Clifton, N.J.)
|February 2, 2026
PubMed
まとめ

レーザーキャプチャーマイクロダイセクションと酵素的メチル化シーケンス（EM-seq）を組み合わせて、希少細胞のDNAメチル化を分析しました。この方法は、バイオマーカー発見と個別化医療のために、わずか100個の細胞から高品質のエピジェネティックデータを提供します。

キーワード:
ビスルファイトフリー変換DNAメチル化プロファイリング酵素的メチル化シーケンス低入力ライブラリ調製空間分解メチル化分析

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Laser Capture Microdissection of Drosophila Peripheral Neurons
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Laser Capture Microdissection of Drosophila Peripheral Neurons

Published on: May 24, 2010

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Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
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Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

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関連する実験動画

Last Updated: Feb 4, 2026

Laser Capture Microdissection of Mammalian Tissue
16:34

Laser Capture Microdissection of Mammalian Tissue

Published on: October 1, 2007

17.5K
Laser Capture Microdissection of Drosophila Peripheral Neurons
12:02

Laser Capture Microdissection of Drosophila Peripheral Neurons

Published on: May 24, 2010

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Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
09:20

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

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科学分野:

  • エピジェネティクス
  • 分子生物学
  • ゲノミクス

背景:

  • 組織の不均一性はDNAメチル化解析を混乱させます。
  • レーザーキャプチャーマイクロダイセクション（LCM）は特定の細胞集団を分離しますが、DNA量が少ないです。
  • 従来のビスルファイトシーケンシングは高入力DNAを必要とし、分解を引き起こします。

主な方法:

  • レーザーキャプチャーマイクロダイセクション（LCM）と酵素的メチル化シーケンス（EM-seq）の統合。
  • 酵素的DNA変換のための低入力ライブラリ調製プロトコルの最適化。
  • DNAの完全性を維持し、GCバイアスを低減するためのビスルファイトフリーメチル化プロファイリング。

結論:

  • LCM-EM-seqは、空間分解エピジェネティック解析のためのスケーラブルなフレームワークです。
  • 希少細胞集団のメチル化を考慮した研究を可能にします。
  • バイオマーカー発見、標的療法、および個別化エピジェネティックプロファイリングを進歩させます。