免疫蛍光イメージング誘導レーザーマイクロダイセクションによる超低入力空間組織プロテオミクス
Sonja Fritzsche1,2, Fabian Coscia3
1Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Spatial Proteomics Group, Berlin, Germany.
Abstract:
Laser microdissection (LMD) enables the precise isolation of specific cells or regions of interest from tissue sections, guiding downstream molecular analyses with high spatial resolution. In this protocol, we describe an optimized protocol combining whole-slide immunofluorescence imaging, LMD, and low-input liquid chromatography (LC) mass spectrometry (MS)-based proteomics of FFPE tissue sections.
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