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Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Multi-input and Multi-variable systems01:22

Multi-input and Multi-variable systems

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Cruise control systems in cars are designed as multi-input systems to maintain a driver's desired speed while compensating for external disturbances such as changes in terrain. The block diagram for a cruise control system typically includes two main inputs: the desired speed set by the driver and any external disturbances, such as the incline of the road. By adjusting the engine throttle, the system maintains the vehicle's speed as close to the desired value as possible.
In the absence of...
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Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Depth Perception and Spatial Vision01:15

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Depth perception is the ability to perceive objects three-dimensionally. It relies on two types of cues: binocular and monocular. Binocular cues depend on the combination of images from both eyes and how the eyes work together. Since the eyes are in slightly different positions, each eye captures a slightly different image. This disparity between images, known as binocular disparity, helps the brain interpret depth. When the brain compares these images, it determines the distance to an object.
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Drugs administered through various routes can lead to nonlinear elimination, resulting in complex pharmacokinetic behaviors crucial to understanding efficacious drug dosing.
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Tissues01:18

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Cells with similar structure and function are grouped into tissues. A group of tissues with a specialized function is called an organ. There are four main types of tissue in vertebrates: epithelial, connective, muscle, and nervous.
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Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment
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免疫蛍光イメージング誘導レーザーマイクロダイセクションによる超低入力空間組織プロテオミクス

Sonja Fritzsche1,2, Fabian Coscia3

  • 1Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Spatial Proteomics Group, Berlin, Germany.

Methods in molecular biology (Clifton, N.J.)
|February 2, 2026
PubMed
まとめ

本研究では、免疫蛍光イメージングと質量分析を組み合わせたレーザーマイクロダイセクション（LMD）の新しいプロトコルを紹介します。この方法により、ホルマリン固定パラフィン包埋（FFPE）組織の特定の細胞を正確にプロテオーム解析することが可能になります。

キーワード:
深層視覚プロテオミクス免疫蛍光染色レーザーマイクロダイセクション組織プロテオミクス

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Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
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Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules
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科学分野:

  • 生物医学研究
  • プロテオミクス
  • 組織分析

背景:

  • レーザーマイクロダイセクション（LMD）は、分子解析のために組織切片から特定の細胞を単離するために重要である。
  • 正確な下流解析には高い空間分解能が必要である。
  • ホルマリン固定パラフィン包埋（FFPE）組織は広く使用されているが、分子研究には困難が伴う。

主な方法:

  • FFPE組織切片に対して全スライド免疫蛍光イメージングを実施した。
  • 関心領域を単離するためにレーザーマイクロダイセクション（LMD）を使用した。
  • 単離されたサンプルに低入力液体クロマトグラフィー（LC）質量分析（MS）ベースのプロテオミクスを適用した。

結論:

  • この最適化されたプロトコルは、高空間分解能を持つFFPE組織の詳細なプロテオーム解析を容易にする。
  • この方法は、細胞の不均一性およびバイオマーカー発見の研究に価値がある。
  • 分子病理学および精密医療研究を進歩させる。