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Quantitative Analysis01:12

Quantitative Analysis

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Quantitative analysis is a technique for measuring the amount of specific constituents in a sample. When the sample's composition is unknown, qualitative analysis is performed first to identify its components, which ensures that the correct substances are measured during the quantitative phase.
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the...
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Quantitative Aspects of Drug-Receptor Interaction01:30

Quantitative Aspects of Drug-Receptor Interaction

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The receptor occupancy theory connects a drug's response to the number of occupied receptors. With higher drug concentrations, more receptors are occupied, leading to increased responses. The formation of drug-receptor complexes involves association and dissociation rates, which reach equilibrium when the forward and backward reactions are equal. The equilibrium association constant (Ka) and its inverse, the equilibrium dissociation constant (Kd), indicate drug affinity. Higher Ka and lower...
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Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

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Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
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Genomics02:02

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Case Studies01:22

Case Studies

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There are many research methods available to psychologists in their efforts to understand, describe, and explain behavior and the cognitive and biological processes that underlie it.
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In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
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Updated: Feb 4, 2026

Laser Capture Microdissection of Drosophila Peripheral Neurons
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レーザーキャプチャーマイクロダイセクションを用いた定量ショットガンリピドミクス

Silvia Radrezza1, HongKee Moon1, Ivey Sebastian1

  • 1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.

Methods in molecular biology (Clifton, N.J.)
|February 2, 2026
PubMed
まとめ

本研究は、特定の組織領域における脂質分子のマッピング方法を導入するものです。レーザーキャプチャーマイクロダイセクションと質量分析法を用いて、脂質組成と機能を明らかにします。

キーワード:
レーザーキャプチャーマイクロダイセクション質量分析法ショットガンリピドミクス空間リピドミクス

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Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

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関連する実験動画

Last Updated: Feb 4, 2026

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科学分野:

  • リピドミクス
  • 分子生物学
  • 組織学

背景:

  • 脂質は組織や器官内に不均一に分布しています。
  • 現在の組織学的方法は、分子特異性と機能的洞察を欠いています。
  • 組織の脂質組成を理解することは、生物学的機能にとって重要です。

研究 の 目的:

  • 空間的に定義された組織領域における脂質分子種の定量方法を開発すること。
  • 数百の脂質種のモル存在量の決定を可能にすること。
  • リピドーマプロファイルを組織構造と潜在的な生物学的役割に関連付けること。

主な方法:

  • レーザーキャプチャーマイクロダイセクション（LCM）とショットガン質量分析（MS）を組み合わせること。
  • 組織凍結切片から特定の領域を空間的に分離すること。
  • これらの分離された領域内の脂質の分子組成を分析すること。

主要な成果:

  • 多数の脂質分子種のモル存在量を決定しました。
  • 膜脂質と中性脂質を含む主要な脂質クラスをカバーしました。
  • 限られた組織領域の詳細なリピドーマ解析を可能にしました。

結論:

  • 記述された方法は、組織における高解像度脂質マッピングを可能にします。
  • この技術は、組織の脂質不均一性に関する分子的な洞察を提供します。
  • 特定の生物学的文脈における脂質機能の理解を促進します。