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Updated: Feb 4, 2026

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Published on: July 7, 2010

ゲノムワイド関連解析により、シイタケのトリコデルマに対する抵抗性を高めるα/βヒドロラーゼ遺伝子LeABHを同定

Yan Xia¹, Wenbing Gong², Liping Yang¹

¹State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, Hubei, China.

Environmental microbiology

|February 2, 2026

Abstract:

Green mould disease caused by Trichoderma spp. severely threatens Lentinula edodes cultivation, resulting in heavy yield losses. This study developed a quantifiable image-based evaluation method using Trichoderma infection rate (TIR) to assess L. edodes resistance. By quantifying TIR in a shiitake association panel, we performed a genome-wide association study (GWAS) and identified 239 SNPs and 245 nearby genes. Among these genes, a subset involved in cell membrane integrity maintenance, substance transport and environmental stress response may be associated with resistance to Trichoderma. Notably, three single-nucleotide polymorphisms (SNPs) on chromosomes 3, 9 and 10 were stably associated with multiple TIR traits. Most importantly, an α/β hydrolase gene, LeABH, was prioritised and functionally validated. Overexpression of LeABH in L. edodes significantly enhanced resistance by 26.7%-33.4% against T. harzianum and by 21.4%-49.7% against T. atroviride, and inhibited conidiation. These results confirmed LeABH as a key causal gene underlying L. edodes resistance to Trichoderma. Taken together, our findings have provided new insights into the genetic basis of L. edodes resistance to Trichoderma. The improved resistance evaluation method, along with the identified genetic variations and candidate genes, has offered valuable resources for the molecular marker-assisted breeding of disease-resistant L. edodes strains.

キーワード:

レンティヌラ・エドデストリコデルマゲノムワイド関連解析抵抗性評価 α/βヒドロラーゼ遺伝子

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Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR

Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR

Published on: November 23, 2012

Large-Scale Multi-Omics Genome-Wide Association Studies Mo-GWAS: Guidelines for Sample Preparation and Normalization

Large-Scale Multi-Omics Genome-Wide Association Studies Mo-GWAS: Guidelines for Sample Preparation and Normalization

Published on: July 27, 2021

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