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Updated: Feb 4, 2026

High Throughput Microinjections of Sea Urchin Zygotes
Published on: January 21, 2014
ウニ大量死の原因となるスキトコチリア科病原体Philaster sp. FWC2株の遺伝的特徴
Shen Jean Lim1, Mya Breitbart1
1College of Marine Science, University of South Florida, St. Petersburg, Florida, USA.
Abstract:
A scuticociliate most closely related to Philaster apodigitiformis caused mass mortalities of diadematoid sea urchins and was cultured as Philaster sp. isolate FWC2. We sequenced the metagenomic content of this isolate, which was predicted to represent ≤ 56% of the complete genome. Based on k-mer counts, the haploid genome size was predicted to be 122-136 Mbp. We assembled and annotated a 4,088 bp nuclear ribosomal operon, a 41,396 bp mitochondrial genome with 19.22% G + C content, 24 protein-coding genes, 6 tRNA genes, and 2 rRNA genes, and a protein sequence homologous to β-PKA in Philaster apodigitiformis potentially involved in host infection.
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