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Atomic Force Microscopy01:08

Atomic Force Microscopy

4.5K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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Leveling Effect and Non-Aqueous Acid-Base Solutions02:11

Leveling Effect and Non-Aqueous Acid-Base Solutions

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This lesson defines the leveling effect in acidic and basic solutions and its role in aqueous and non-aqueous solutions. It is essential to understand the competing nature of various species in a chemical system.
The Leveling Effect of a Solvent
A generic acid (HA) reacts with the generic base (B-) to yield the corresponding conjugate base (A-) and conjugate acid (HB):
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Chemical Reactions in Aqueous Solutions03:03

Chemical Reactions in Aqueous Solutions

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Chemical substances interact in many different ways. Certain chemical reactions exhibit common patterns of reactivity. Due to the vast number of chemical reactions, it becomes necessary to classify them based on the observed patterns of interaction.
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Transmission-based Precautions II: Airborne and Protective Environment01:25

Transmission-based Precautions II: Airborne and Protective Environment

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Transmission-based precautions are for patients infected or suspected to be infected (or colonized) with organisms posing a significant risk to others. The transmission precautions include airborne and protective environment precautions.
Airborne precautions:
Use airborne precautions when treating patients known or suspected to have diseases that spread through the air—for example, tuberculosis or measles. These organisms are present in smaller droplets expelled by an infected person and...
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Acids, Bases and Neutralization Reactions03:26

Acids, Bases and Neutralization Reactions

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An acid-base reaction is one in which a hydrogen ion, H+, is transferred from one chemical species to another. Such reactions are of central importance to numerous natural and technological processes, ranging from the chemical transformations within cells or lakes and oceans to the industrial-scale production of fertilizers, pharmaceuticals, and other substances essential to the society.
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Atomic Orbitals02:44

Atomic Orbitals

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An atomic orbital represents the three-dimensional regions in an atom where an electron has the highest probability to reside. The radial distribution function indicates the total probability of finding an electron within the thin shell at a distance r from the nucleus. The atomic orbitals have distinct shapes which are determined by l, the angular momentum quantum number. The orbitals are often drawn with a boundary surface, enclosing densest regions of the cloud.
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Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
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水環境におけるグラフェンベースのマイクロ流体セルを用いた原子間力顕微鏡ベースの光熱赤外顕微鏡検査

Yasuhiko Fujita1, Mariko Takahashi1, Hirohmi Watanabe1

  • 1Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST) Kagamiyama 3-11-32 Higashihiroshima 739-0046 Japan Yasuhiko.fujita@aist.go.jp.

Nanoscale advances
|February 4, 2026
PubMed
まとめ

水和ポリマーを水中、原子間力顕微鏡ベースの光熱誘起共鳴（PTIR）を用いて実証します。この技術は、PTIRスペクトルの変化を分析することによってポリマーの膨潤を検出し、ポリマーの挙動に関する新たな洞察を提供します。

キーワード:
原子間力顕微鏡光熱誘起共鳴赤外顕微鏡水和ポリマーマイクロ流体グラフェンポリマー膨潤分光法

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科学分野:

  • 材料科学
  • 高分子化学
  • 分光法

背景:

  • 水性環境における水和ポリマーの研究は、従来の特性評価技術にとって課題となっています。
  • 原子間力顕微鏡（AFM）は高解像度の表面イメージングを提供しますが、液体中での分光法との統合は複雑です。

研究 の 目的:

  • AFMベースの光熱誘起共鳴（PTIR）を用いた水中水和ポリマーの分析のための新しい方法を開発および実証すること。
  • PTIRが水性条件下での膨潤などのポリマー構造の変化を検出する可能性を調査すること。

主な方法:

  • 原子層レベルのグラフェン層を赤外線透過窓として使用したマイクロ流体セルを利用しました。
  • マイクロ流体セル内の水和ポリマーに対して光熱誘起共鳴（PTIR）測定を実施しました。
  • スペクトル変化を分析してポリマーの膨潤を特定しました。

主要な成果:

  • 水和ポリマーに対するPTIR測定を水性環境で実証しました。
  • ポリマーの膨潤と相関するPTIRスペクトルの明確な変化を観察しました。
  • マイクロ流体PTIRのための効果的な赤外線透過窓としてグラフェンの使用を検証しました。

結論:

  • AFMベースのPTIRは、水溶液中の水和ポリマーを特性評価するための実行可能な技術です。
  • PTIRはスペクトルシフトを分析することによってポリマーの膨潤を効果的に検出できます。
  • この方法は、生物学的に関連性のある条件下でのポリマーのダイナミクスと相互作用を研究するための新しい道を開きます。