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Updated: Feb 6, 2026

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

Published on: October 22, 2012

可溶性抗原アレイは、エピトープ特異的糖尿病原性T細胞の応答を変化させる

Grant M Downes¹, Camillo Bechi Genzano², Colin R Silva^3,4

¹Bioengineering Graduate Program, University of Kansas, Lawrence, Kansas 66045, United States.

ACS applied bio materials

|February 4, 2026

Abstract:

Type 1 diabetes (T1D) is driven by autoreactive T cells, which destroy insulin-producing β cells. Antigen-specific immunotherapies (ASITs) aim to restore immune tolerance while avoiding the broad immunosuppression of current therapies. Soluble antigen arrays (SAgAs) are multivalent antigen constructs built on a low-molecular-weight hyaluronic acid (HA) backbone, previously validated in preclinical models. Here, we generated SAgAs displaying human T1D autoantigen epitopes, including Insulin B:9-23 (InsB:9-23) and a hybrid insulin peptide (HIP), alongside a hemagglutinin (HAg):306-318 control. We confirmed their structure, valency, and ability to engage human epitope-specific T cell clones. Both InsB:9-23-SAgA and HIP-SAgA demonstrated strong specificity, supporting their utility as T cell probes and potential candidates for ASIT in T1D.

キーワード:

抗原特異的免疫療法糖尿病原性T細胞エピトープ特異的ターゲティングヒアルロン酸複合体可溶性抗原アレイ 1型糖尿病

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The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo

The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo

Published on: June 19, 2014

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

Published on: October 20, 2021

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