細胞遺伝子導入および単一細胞ナノ振動動態モニタリングのためのナノセンサープラットフォーム

Cell transfection and dynamic monitoring of subsequent cellular physiological activity play a crucial role in studying gene function and regulating gene expression. However, platforms integrating cell transfection with dynamic monitoring of cellular physiological activity are rarely reported. Here, we propose a novel technology using a nanopore array platform combined with nanodrums fabricated from ultrathin graphene, which enables single-cell transfection while dynamically monitoring post-transfection changes in cellular physiological activity. In our exploration, we found that random oscillation amplitudes generated by single OCI-Ly3 cells reached up to ∼20 nm. After transfection with the ME2 gene knockdown sequence (shME2), cellular physiological activity was inhibited, and the random oscillations were suppressed, with amplitudes decreasing to < 5 nm. Our results provide a high-throughput potential and efficient approach for gene transfection and dynamic monitoring of cellular physiological activity, offering valuable insights for cell-based drug screening, cancer cell subtype classification, and other applications.