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Updated: Feb 6, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

ハイブリッド単為生殖クルミ属複合体における残存組換えのゲノム的影響

Wei-Ping Zhang^1,2,3, Sylvain Glémin^3,4, Xiao-Xu Pang²

¹State Key Laboratory of Plant Diversity and Specialty Crops, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.

Nature communications

|February 4, 2026

Abstract:

Apomixis, a form of clonal asexual reproduction in plants, is often accompanied by residual sex, yet its genomic consequences remain poorly understood. Here, we assembled a haplotype-resolved genome of Carya hunanensis and analyzed whole-genome resequencing data from 195 adults and 180 mature embryos across four hickory species, representing a hybrid apomictic complex with both sexual and asexual lineages. We find apomictic species exhibited genomic signatures of clonality, notably loss of heterozygosity (LOH), suggesting recombination induced by rare sexual events. Despite harboring more heterozygous deleterious variants, apomictic adults showed lower realized mutation loads, particularly in hybrid C. hunanensis, whose apomictic haplotype disproportionately carried deleterious alleles. Remarkably, rare embryos from apomicts underwent recombination-mediated LOH, exposing deleterious mutations to selection. These findings reveal the genetic cost of residual sex, while also indicating its role in generating novel genotypes, supported by close relatedness among adult apomicts. Our study provides a unique genomic snapshot of how residual sex and recombination mitigate mutation accumulation and potentially facilitate clonal maintenance in natural asexual systems.

キーワード:

単為生殖組換え突然変異負荷ゲノムクルミ属

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Array Comparative Genomic Hybridization Array CGH for Detection of Genomic Copy Number Variants

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Published on: February 21, 2015

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

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