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Updated: Feb 7, 2026

Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor

Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor

Published on: April 1, 2011

プレートリーダーベースの遺伝子コード化カルシウムインジケーターGCaMPを用いた免疫応答における細胞カルシウム動態の定量化

Hyunsuh Lee¹, Robin Hon Ming Goh¹, Wolfgang Moeder¹

¹Department of Cell & Systems Biology, University of Toronto, Toronto, ON, Canada.

Methods in molecular biology (Clifton, N.J.)

|February 5, 2026

Abstract:

Calcium ions (Ca²⁺) serve as crucial secondary messengers in numerous signaling cascades in plants, similar to their role in animals. One of the earliest responses in the activation of immune responses is a rapid, transient increase in cytosolic Ca²⁺ ([Ca²⁺]_cyt). Measuring the spatial and temporal changes in [Ca²⁺]_cyt is essential for understanding the details of immune responses to various pathogens and pests. We present a straightforward assay for measuring [Ca²⁺]_cyt using the genetically encoded Ca²⁺ indicator, GCaMP, and a fluorescence microplate reader. This assay allows for the assessment of changes in [Ca²⁺]_cyt with high temporal resolution following various elicitor treatments. Additionally, we describe a method to analyze [Ca²⁺]_cyt using a dexamethasone-inducible effector expression system (proDEX::AvrRpm1). Due to the simplicity of these methods, they are also suitable for high-throughput screening.

キーワード:

カルシウムシグナル ETI エフェクターエリシター GCaMP 遺伝子コード化カルシウムインジケーター免疫応答 PTI プレートリーダー

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Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators

Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators

Published on: November 19, 2014

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