関連する実験動画

Updated: Feb 7, 2026

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

Published on: October 22, 2012

ケミルミネッセンスを利用した植物免疫NADPHオキシダーゼによる細胞間隙ROSの可視化と定量化

Miki Yoshioka¹, Hirofumi Yoshioka²

¹Laboratory of Plant Immunology, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Aichi, Japan.

Methods in molecular biology (Clifton, N.J.)

|February 5, 2026

Abstract:

Reactive oxygen species (ROS) are implicated in plant innate immunity. When plants recognize pathogens, apoplastic superoxide (O₂^-) is produced by a plasma membrane NADPH oxidase (RBOH; Respiratory Burst Oxidase Homolog), which is immediately dismutated to hydrogen peroxide (H₂O₂). Nicotiana benthamiana NbRBOHB is responsible for both the transient pattern-triggered immunity (PTI)-ROS burst and the robust effector-triggered immunity (ETI)-ROS burst. Potato (Solanum tuberosum) StRBOHB and StRBOHC are phosphorylated by potato calcium-dependent protein kinases, StCDPK4 and StCDPK5, resulting in the ROS burst. The ROS contribute to the potato resistance to a virulent isolate of late blight pathogen, Phytophthora infestans. Here, we introduce an apoplastic ROS detection protocol utilizing L-012-derived chemiluminescence in response to Agrobacterium-mediated co-expression of StRBOHC and StCDPK5 in N. benthamiana leaves.

キーワード:

細胞間隙ROS カルシウム依存性プロテインキナーゼケミルミネッセンス L-012 NADPHオキシダーゼ RBOH

さらに関連する動画

Author Spotlight: Optimizing Apoplast Protein Extraction for Efficient Recovery of Recombinant Proteins from Plant Cells

Author Spotlight: Optimizing Apoplast Protein Extraction for Efficient Recovery of Recombinant Proteins from Plant Cells

Published on: July 5, 2024

Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay

Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay

Published on: November 25, 2022

関連する概念動画

The Apoplast and Symplast

The Apoplast and Symplast

Plant growth depends on its ability to take up water and dissolved minerals from the soil. The root system of every plant is equipped with the necessary tissues to facilitate the entry of water and solutes. The plant tissues involved in the transport of water and minerals have two major compartments - the apoplast and the symplast. The apoplast includes everything outside the plasma membrane of living cells and consists of cell walls, extracellular spaces, xylem, phloem, and tracheids. The...

Plant Breeding and Biotechnology

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

What is the Immune System?

What is the Immune System?

Plant Hormones

Plant Hormones

Plant hormones—or phytohormones—are chemical molecules that modulate one or more physiological processes of a plant. In animals, hormones are often produced in specific glands and circulated via the circulatory system. However, plants lack hormone-producing glands.

Tonicity in Plants

Tonicity in Plants

Tonicity describes the capacity of a cell to lose or gain water. It depends on the quantity of solute that does not penetrate the membrane. Tonicity delimits the magnitude and direction of osmosis and results in three possible scenarios that alter the volume of a cell: hypertonicity, hypotonicity, and isotonicity. Due to differences in structure and physiology, tonicity of plant cells is different from that of animal cells in some scenarios.

Plant Cell Wall

Plant Cell Wall

The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.