人工酵素スクリーニング・機能変換プラットフォームEnzySFC：ニトリラーゼからニトリルヒドラターゼへの機能完全転換
Shuiqin Jiang1, Zhelin Zheng1, Hua Dong1
1Research Center for Systems Biosynthesis, National Key Laboratory of Lead Draggability Research, China State Institute of Pharmaceutical Industry, Gebaini Road 285, Shanghai 201203, China.
Journal of agricultural and food chemistry
|February 6, 2026
まとめ
AIプラットフォームであるEnzySFCは、酵素の活性と特異性を向上させるために酵素を発見・進化させます。アミド生産能力の高いニトリラーゼを特定し、酵素エンジニアリングを加速しました。
科学分野:
- 生化学; 酵素工学; 計算生物学
背景:
- 触媒的寛容性は酵素進化の理解の鍵となります。複数の機能を持つ酵素を発見し進化させることは、大きな課題を提示します。
研究 の 目的:
- 新規酵素発見と機能進化のためのAI支援プラットフォームEnzySFCを開発すること。多目的エンジニアリングのための酵素活性と特異性の協調的最適化を可能にすること。
主な方法:
- EnzySFCは、新規酵素発見と機能進化を統合します。1113の候補ニトリラーゼの実験的検証。ニトリラーゼ変異体のAI主導の進化。
主要な成果:
- 1113の未解明なニトリラーゼのうち10が実験的に検証されました。検証されたニトリラーゼの90%が触媒活性を示し、80%がアミド形成を示しました。AI主導の進化により16の検証済みニトリラーゼ変異体が得られ、4つは100%のアミド収率と完全な基質変換を達成しました。
結論:
- EnzySFCは、多目的酵素エンジニアリングのための移転可能なフレームワークを提供します。このプラットフォームは、効率的な酵素触媒の開発を加速します。
関連する概念動画
02:45Functional Groups
89.0K
Functional groups are a group of atoms with characteristic properties, which when linked to the carbon skeleton of a molecule, alter the properties of that molecule. For example, the presence of certain functional groups on a molecule will make them hydrophilic, whereas others will make them hydrophobic. These functional groups are an indispensable part of organic chemistry and important components of biological molecules, such as carbohydrates, proteins, lipids, and nucleic acids. Each...
89.0K
02:45Functional Groups
24.5K
24.5K
02:08Gene Conversion
10.7K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
10.7K
01:58Mechanical Protein Functions
5.6K
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force.
5.6K
01:11Functionalism
2.6K
William James, John Dewey, and Charles Sanders Peirce were instrumental in founding functional psychology, which draws heavily from Darwin's theory of evolution by natural selection. This theory suggests that individual traits, including behaviors, are adapted to their environments through natural selection. At the heart of functionalism is the concept of adaptation, meaning that a trait enhances an individual's chances of survival and reproduction.
James envisioned psychology's...
James envisioned psychology's...
2.6K
01:56Structural Protein Function
30.0K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
30.0K