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Updated: Feb 8, 2026

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

Published on: May 2, 2017

単一マイクロRNA miR-195はEBF1欠損によって誘発されるB細胞発生の停止を救済する

Yuji Miyatake¹, Takeshi Kamakura², Tomokatsu Ikawa³

¹Department of Innovative Medical Science, Tokai University School of Medicine, Isehara, Japan.

|February 6, 2026

Abstract:

Accumulated studies have reported that hematopoietic differentiation was primarily regulated by transcription factors. Early B cell factor 1 (EBF1) is an essential transcription factor for B lymphopoiesis. Contrary to the canonical notion, we found that a single miRNA, miRNA-195 (Mir195) transduction let Ebf1-deficient hematopoietic progenitor cells (HPCs) express CD19, carry out V(D)J recombination and class switch recombination, which implied that B cell matured without EBF1. A part of the mechanism was caused by FOXO1 accumulation via inhibition of FOXO1 phosphorylation pathways in which targets of Mir195 are enriched. These results suggested that some miRNA transductions could function as alternatives to transcription factors.

キーワード:

B細胞発生 EBF1 FOXO1 Mir195 免疫学炎症マウス

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A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

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Single-cell Profiling of Developing and Mature Retinal Neurons

Single-cell Profiling of Developing and Mature Retinal Neurons

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