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Updated: Feb 8, 2026

Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements

Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements

Published on: July 29, 2021

ナノバブルの気液界面における空気からの亜硝酸塩および硝酸塩の高効率生産

Sandeep Bose¹, Saeed Bahadorikhalili², Yuanyi He¹

¹Department of Chemistry, Stanford University, Stanford, CA 94305, USA.

Science advances

|February 6, 2026

Abstract:

We report a simple, one-step conversion of air to nitrogen oxyanions (NO_x^-), i.e., nitrate (NO₃^-) and nitrite (NO₂^-), at the gas-water interface (GWI) of nanobubbles (NBs). The nanobubble generator is placed inside an aqueous solution of 50 μM Fe²⁺ to enhance the production of hydroxyl radicals (OH•) by initiating Fenton's reaction at the GWI. The formation of NO_x^- does not require any external potential or radiation. The NO_x^- production rate using NBs is found to be 60.4 ± 1.21 μM hour^-1, which shows a fourfold increase in the production as compared to the same reaction performed in microbubbles (15 μM hour^-1), which is the result from an enhanced electric field strength at the GWI. We propose that this nitrogen-fixation approach presents a promising pathway for an eco-friendly, energy-efficient, and scalable solution for NO_x^--based sustainable fertilizer production.

キーワード:

ナノバブル窒素固定窒素オキシアニオン肥料生産環境化学グリーンケミストリー

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