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Updated: Feb 9, 2026

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

cfDNA分子末端のホリスティックな決定

Peiyong Jiang¹, Mary-Jane L Ma², Rong Qiao²

¹Centre for Novostics, Hong Kong Science Park, Pak Shek Kok, New Territories, Hong Kong SAR, China; Li Ka Shing Institute of Health Sciences, the Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China; Department of Chemical Pathology, the Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China; State Key Laboratory of Translational Oncology, the Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong SAR, China.

|February 7, 2026

Abstract:

Cell-free DNA (cfDNA) end motifs serve as fragmentomics biomarkers for cancer. Prior studies primarily focused on 5' ends, whereas 3' ends were overlooked due to artifactual modification in existing sequencing protocols. We utilized single-stranded library preparation ("2-end sequencing") to assess the native 5' and 3' end motifs (EM5 and EM3, respectively). Additionally, we demonstrated diagnostic power from the nucleotide motifs located immediately upstream and downstream of 5' and 3' ends, named pre-end motifs (PREMs) and post-end motifs (POEMs). These fragmentomics markers collectively achieved an area under the curve (AUC) of 0.95 for hepatocellular carcinoma (HCC) detection. Fragmentomics-based methylation analysis of 3' ends (3' FRAGMA) improved detection of HCC (AUC: 0.97). We further developed "4-end sequencing" to interrogate both ends of both strands of a double-stranded cfDNA molecule, enhancing fragmentomics-based cancer detection. Holistic end profiling adds to the armamentarium of liquid biopsy and sheds light on the biology of cfDNA fragmentation.

キーワード:

がん検出末端モチーフフラグメントミクス非侵襲的

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