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関連する概念動画

Ions and Ionic Charges03:27

Ions and Ionic Charges

79.3K
In ordinary chemical reactions, the nucleus — which contains the protons and neutrons of each atom and thus identifies the element — remains unchanged. Electrons, however, can be added to atoms by transfer from other atoms, lost by transfer to other atoms, or shared with other atoms. The transfer and sharing of electrons among atoms govern the chemistry of the elements. During the formation of some compounds, atoms gain or lose electrons to form electrically charged particles called...
79.3K
Ion Channels01:19

Ion Channels

91.5K
The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow...
91.5K
Common Ion Effect03:24

Common Ion Effect

47.0K
Compared with pure water, the solubility of an ionic compound is less in aqueous solutions containing a common ion (one also produced by dissolution of the ionic compound). This is an example of a phenomenon known as the common ion effect, which is a consequence of the law of mass action that may be explained using Le Châtelier’s principle. Consider the dissolution of silver iodide:
47.0K
Precipitation of Ions03:11

Precipitation of Ions

30.3K
Predicting Precipitation
The equation that describes the equilibrium between solid calcium carbonate and its solvated ions is:
30.3K
Formation of Complex Ions03:45

Formation of Complex Ions

26.2K
A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
26.2K
Ions as Acids and Bases02:54

Ions as Acids and Bases

26.6K
Salts with Acidic Ions
Salts are ionic compounds composed of cations and anions, either of which may be capable of undergoing an acid or base ionization reaction with water. Aqueous salt solutions, therefore, may be acidic, basic, or neutral, depending on the relative acid-base strengths of the salt’s constituent ions. For example, dissolving the ammonium chloride in water results in its dissociation, as described by the equation:
26.6K

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Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
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イオン移動度分解質量分析法によるディープカバレッジ単一細胞メタボロミクス

Mingdu Luo1,2, Tianzhang Kou1,2, Yandong Yin1

  • 1Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, P. R. China.

Nature methods
|February 9, 2026
PubMed
まとめ

研究者らは、感度とカバレッジを強化した新しい単一細胞メタボロミクス技術を開発した。この画期的な技術により、細胞代謝と不均一性に関するより深い洞察が可能になり、既存の方法が改善される。

キーワード:
単一細胞メタボロミクス質量分析法イオン移動度代謝プロファイリング細胞不均一性

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Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage
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Thermochemical Studies of NiII and ZnII Ternary Complexes Using Ion Mobility-Mass Spectrometry
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Thermochemical Studies of NiII and ZnII Ternary Complexes Using Ion Mobility-Mass Spectrometry

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科学分野:

  • 生化学
  • 分析化学
  • 細胞生物学

背景:

  • 現在の単一細胞メタボロミクス法は、感度、堅牢性、および代謝物質カバレッジに限界があります。
  • 単一細胞レベルでの包括的な代謝プロファイリングを可能にする高度な技術が必要です。

研究 の 目的:

  • 多次元単一細胞メタボロームプロファイリングのための新しいイオン移動度分解質量分析技術を導入すること。
  • 単一細胞メタボロミクスの感度、堅牢性、および代謝物質カバレッジを強化すること。

主な方法:

  • 高スループット単一細胞注入とイオン移動度質量分析の統合。
  • イオン移動度を利用した選択的イオン蓄積と細胞重ね合わせベースの増幅の実装。
  • データ解析には計算ツールMetCellを使用しました。

主要な成果:

  • 個々の細胞内での代謝物質検出において、アトモルレベルの感度と広いダイナミックレンジを達成しました。
  • 細胞あたり約800の代謝物質をアノテーションし、5,000以上の代謝ピークを検出し、既存の方法と比較して3倍から10倍の改善を達成しました。
  • 45,603個の一次肝細胞に適用し、正確な細胞アノテーションを可能にし、老化肝細胞における代謝的不均一性を明らかにしました。

結論:

  • 開発された技術は、ハイスループット単一細胞メタボロミクスの新しいベンチマークを設定します。
  • このプラットフォームは、単一細胞解像度での代謝的不均一性の理解を大幅に進歩させます。
  • 深い代謝的洞察を必要とする生物学的研究のための強力なツールを提供します。