関連する実験動画

Updated: Feb 11, 2026

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

生細胞およびゼブラフィッシュにおける第一鉄イオンイメージングのための、迅速かつ生体適合性のあるターンオン型NIRプローブ

Leqin Cheng¹, Boxing Geng¹, Rui Wang²

¹School of Chemistry and Pharmaceutical Engineering, Jilin University of Chemical Technology, JilinJilin, 132022, China.

Analytical and bioanalytical chemistry

|February 9, 2026

Abstract:

Leveraging an ICT-controlled turn-on mechanism, we developed a near-infrared fluorescent probe (E)-2-bromo-4-(2-(4-(dicyanomethylene)-4H-chromen-2-yl)vinyl)-N,N-dimethylaniline oxide, hereinafter denoted as DCMB, built on a propanedinitrile-benzopyran scaffold for the specific detection of ferrous ions (Fe²⁺). The probe exhibits a remarkable fluorescence enhancement upon exposure to Fe²⁺, achieving a detection limit of 5.6 µM and a rapid response within 90 s. Strategic incorporation of bromine substituents extends the operational pH window to 5.0-7.5, outperforming most conventional indicators and ensuring robust performance in both physiological milieus and acidic tumor microenvironments. In addition, DCMB displays exceptional selectivity for Fe²⁺ over abundant biological metal ions (Ca²⁺, Mg²⁺, etc.) and potential interfering substances in biological systems, showing no significant effect. It is well-suited for detecting Fe²⁺ in complex biological matrices and has been successfully applied for imaging in living cells and zebrafish employed for in vivo.

キーワード:

Fe2+検出蛍光プローブ近赤外イメージングターンオンセンサー

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