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Updated: Feb 11, 2026

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

Published on: March 19, 2010

細胞の空間的配置の制御：表面マイクロピラーパターンを用いたゴルジ形態および機能の操作

Juul Verbakel¹, Jan de Boer¹

¹Department of Biomedical Engineering and Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, the Netherlands.

Journal of cell science

|February 10, 2026

Abstract:

Sensing the mechanical environment enables cells to rapidly respond to physical tissue parameters, influencing decisions regarding shape, function, and fate. Despite the well-established effects of physical cues on cellular morphological characteristics, the effect of these cues, including surface topography and confinement, on organelle architecture has not yet been thoroughly investigated. This study aims to identify the Golgi-specific transcriptional program involved in cellular adaptation to pillar-induced confinement using bone marrow-derived mesenchymal stem cells as a model system. We make use of seven polystyrene micro-pillar arrangements with unique designs that induce a high diversity in structural Golgi organization. Transcriptional analysis of functionally relevant Golgi genes shows a unique fingerprint at the gene expression level for each pillar design, and variation in the amount of affected genes can partly be explained by the level of confinement and disruption of Golgi organization respectively. We outline a method using pillar-enhanced substrates as a model system to reveal cellular adaptation to extracellular mechanical cues such as confinement, encouraging further exploration of the effects of physical cues on organelle structural reorganization and functional adaptation.

キーワード:

閉じ込めゴルジ再編成機械的合図マイクロピラー配置転写的再プログラム

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Fabrication of Micro-Patterned Chip with Controlled Thickness for High-Throughput Cryogenic Electron Microscopy

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