CuFe2O4磁性粒子類氧化酶活性無外部空白基質的螢光感測平台用於檢測酪蛋白磷酸肽
Kaixin Liu1, Hongxuan Zhang1, Zhihong Guan1
1Ministry of Education Key Laboratory of Laser Life Science & Guangdong Provincial Key Laboratory of Laser Life Science & Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, China.
Abstract:
Casein Phosphopeptide (CPP), a peptide for calcium absorption enhancement and bone mineralization, is a key nutritional fortification of infant formula. However, rapid and accurate detection of CPP remains challenging. In this study, the first external blank matrix-free, on-site fluorescence sensing platform for CPP is proposed. The method relies on the collaboration of CuFe2O4 magnetic particles (MPs) and TiO2@PDA@Fe3O4 MPs. CuFe2O4 MPs exhibit oxidase-like activity, catalyzing the oxidation of o-phenylenediamine (OPD) and emitting fluorescence, while CPP can inhibit its oxidation. Meanwhile, TiO2@PDA@Fe3O4 MPs can selectively adsorb CPP and phosphate impurities during pretreatment, reducing matrix interference. The platform was verified by unknown milk powder samples and demonstrated excellent selectivity, sensitivity, and accuracy (limit of detection: 1.04 μg mL-1, linear range: 5-100 μg mL-1, recovery rates: 96.2-108.5%, relative standard deviations: < 4.6%). Moreover, a self-designed portable device was constructed, enabling on-site CPP detection.
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