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Updated: Feb 12, 2026

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

Published on: September 9, 2010

NCSTNノックアウトマウス皮膚におけるJAK/STATシグナル伝達分子の発現と免疫組織化学的局在

Zhe-Ye Shi, Xiao-Jie Jin, Shu-Yuan Sun

Clinical laboratory

|February 11, 2026

要旨

Background:

Mutations in the NCSTN gene are believed to be involved in the pathogenesis of acne inversa (AI). Further, certain cytokines are overexpressed in the inflammatory milieu of AI skin. Considering its central role in cytokine regulation, the JAK/STAT signaling pathway should be examined as a pathogenic factor and potential therapeutic target in AI.

Methods:

NCSTNflox/+, CAGGCre-ERTM mice were given 10 mg/kg/day tamoxifen for 6 days. Knockout of the NCSTN gene was confirmed by PCR. The levels of JAK1, JAK2, JAK3, and TYK2 in the skin tissue of NCSTN conditional knockout mice and WT mice were measured by IHC.

Results:

By objective scoring using ImageJ software, JAK2 (p = 0.013) expression was increased in the dermis of KO mice, and JAK1 (p = 0.032), JAK3 (p = 0.028), and TYK2 (p = 0.029) were upregulated in the epidermis of KO mice versus WT mice.

Conclusions:

This study suggests that the JAK/STAT pathway is important in AI, rendering it a potential therapeutic target. Targeting the JAK1/2 may be more prominent in the treatment of HS.

キーワード:

アクネ inversa NCSTN JAK/STAT経路免疫組織化学ヤヌスキナーゼ STAT転写因子

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Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

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