脳脊髄液中のグラム陰性菌の抗生物質感受性：Mueller-Hintonブロスおよび人工脳脊髄液との比較

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Updated: Feb 13, 2026

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

Published on: December 4, 2017

Katrin Biesner¹, Marija Djukic², Utz Reichard³

¹Department of Neuropathology, University Medical Center Göttingen, Göttingen, Germany.

International journal of antimicrobial agents

|February 11, 2026

要旨

Introduction:

Antibiotic resistance of Gram-negative bacteria has become an increasing problem in the therapy of critically ill patients, particularly in central nervous system (CNS) infections. In clinical routine, minimal inhibitory (MICs) and minimal bactericidal concentrations (MBCs) usually are determined in standard test media, not in respective body fluids.

Methods:

MICs and MBCs were tested in human cerebrospinal fluid (CSF), Mueller-Hinton broth (MHB) and artificial cerebrospinal fluid (A-CSF) by microdilution for 24h at 37°C in room air supplemented with 5% CO₂.

Results:

Cefotaxime, ceftazidime and ciprofloxacin inhibited the growth of Pseudomonas aeruginosa, cefotaxime, ceftazidime and amikacin the growth of Morganella morganii, and amikacin the growth of Acinetobacter baumannii in the nutrient-deficient medium CSF at lower concentrations than the MICs determined in MHB. The differences in MIC between MHB and A-CSF were less pronounced than between MHB and human CSF.

Conclusion:

In CNS infections, when antimicrobial susceptibility testing in standard media does not result in adequate therapeutic options, we suggest to test the antimicrobial susceptibility of the causative bacterium in human CSF.

キーワード:

最小発育阻止濃度 Mueller-Hintonブロス人工脳脊髄液脳脊髄液最小殺菌濃度

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