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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

Regulated mRNA Transport

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

Regulated mRNA Transport

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

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関連する実験動画

Updated: May 12, 2026

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

脳組織空間におけるmiRNA制御：3'UTRの視点

Denise Aigner¹, Florian Bartsch¹, Poojashree Bhaskar¹

¹Max-Delbruck-Centrum fur Molekulare Medizin in der Helmholtz-Gemeinschaft.

RNA (New York, N.Y.)

|February 11, 2026

まとめ

本レビューは、空間的トランスクリプトミクスにおけるマイクロRNA（miRNA）とその標的3'非翻訳領域（3'UTR）を探るものである。現在の方法では両者を同時に定量化できず、組織における遺伝子制御の理解が制限されている。

キーワード:

マイクロRNA 空間的トランスクリプトミクス 3'非翻訳領域神経発生遺伝子制御

さらに関連する動画

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

関連する実験動画

Last Updated: May 12, 2026

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

科学分野:

分子生物学
神経科学
ゲノミクス

背景:

マイクロRNA（miRNA）は、生物学的プロセスおよび疾患における重要な遺伝子発現調節因子である。
それらの空間的および時間的発現パターンは、組織内で複雑である。
3'非翻訳領域（3'UTR）には重要なmiRNA標的部位が含まれているが、その空間的バリエーションは十分に研究されていない。

研究の目的:

miRNA媒介遺伝子制御、特に神経発生および神経機能における役割をレビューすること。
miRNAおよび3'UTRの空間的定量化のための既存の方法を要約すること。
この分野における課題を特定し、将来の研究の方向性を提案すること。

主な方法:

空間的トランスクリプトミクスに関する現在の実験技術の議論。
空間RNAデータの解析のための計算アプローチの概要。
in situでのmiRNAおよび3'UTRの同時定量化に適用可能な方法に焦点を当てる。

主要な成果:

空間的トランスクリプトミクスは、サブセルラー分解能でのRNA定量化を可能にする。
現在、miRNAとその標的3'UTRの両方を同時に空間的に定量化できる技術は存在しない。
空間的な3'UTRアイソフォームのバリエーションとその制御的影響に関する重大なギャップが存在する。

結論:

miRNAおよび3'UTRの同時空間定量化は、重要な未解決のニーズである。
このギャップに対処するためには、空間的トランスクリプトミクスツールのさらなる開発が必要である。
空間的miRNA-3'UTR相互作用の理解は、神経科学および疾患における複雑な遺伝子制御を解読するために不可欠である。