Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Formation of Complex Ions03:45

Formation of Complex Ions

26.2K
A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
26.2K
Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

14.9K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
14.9K
Protein Complex Assembly02:41

Protein Complex Assembly

16.9K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.9K
Responses to Salt Stress02:02

Responses to Salt Stress

14.7K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
14.7K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

3.0K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
3.0K
Formation of Species01:31

Formation of Species

45.4K
Speciation describes the formation of one or more new species from one or sometimes multiple original species. The resulting species are discrete from the parent species, and barriers to reproduction will typically exist. There are two primary mechanisms, speciation with and without geographic isolation—allopatric and sympatric speciation, respectively.
45.4K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Fluorescence microscopy imaging and molecular dynamics simulation studies on methylamphetamine solvation fluctuation disturbing lipid bilayer integrity and permeability.

The Journal of chemical physics·2026
Same author

Neuronal Stimulant Dipolar Molecule Solvation Fluctuation in a Nanostructured and Confined Environment of the Lipid Bilayer: A Dynamic Disordered Rate Process Disrupting the Cell Membrane.

The journal of physical chemistry. B·2025
Same author

Insights from Void Volumes and Hydration Dynamics on Protein Spontaneous Rupture via Dynamic Internal Impact Forces: Protein Compressibility Changes Under External Piconewton Compressive Force.

The journal of physical chemistry. B·2025
Same author

Compressive Force Activation of the Neuronal Nitric Oxide Synthase Enzyme.

ACS omega·2025
Same author

Electric Patch-Clamp Probing and Computational Studies of Lipid Bilayer Structural Fluctuations Induced by Methylamphetamine on a Neuronal Cell Membrane.

ACS chemical neuroscience·2025
Same author

Probing the Epidermal Growth Factor Receptor under Piconewton Mechanical Compressive Force Manipulations.

The journal of physical chemistry. B·2025

関連する実験動画

Updated: Feb 13, 2026

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
09:33

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

Published on: March 20, 2018

14.3K

タンパク質活性化複合体形成における酵素活性化複合体形成の誘導

Lalita Shahu1, Dedunu S Senarathne1, Sandipan Saha1

  • 1Center for Photochemical Sciences, Department of Chemistry, Bowling Green State University, Bowling Green, OH 43403, USA. hplu@bgsu.edu.

Physical chemistry chemical physics : PCCP
|February 11, 2026
PubMed
まとめ

分子集合は、カルモジュリン（CaM）と一酸化窒素合成酵素（NOS）の相互作用に大きな影響を与える。この研究により、活性CaM-NOS複合体の形成には、化学的要因だけでなく、高分子集合が重要であることが明らかになった。

キーワード:
分子集合カルモジュリン一酸化窒素合成酵素酵素活性化生化学生物物理学

さらに関連する動画

The Trier Social Stress Test Protocol for Inducing Psychological Stress
09:39

The Trier Social Stress Test Protocol for Inducing Psychological Stress

Published on: October 19, 2011

58.3K
Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes
07:22

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

4.6K

関連する実験動画

Last Updated: Feb 13, 2026

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
09:33

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

Published on: March 20, 2018

14.3K
The Trier Social Stress Test Protocol for Inducing Psychological Stress
09:39

The Trier Social Stress Test Protocol for Inducing Psychological Stress

Published on: October 19, 2011

58.3K
Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes
07:22

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

4.6K

科学分野:

  • 生化学
  • 分子生物学
  • 生物物理学

背景:

  • カルモジュリン（CaM）は、一酸化窒素合成酵素（NOS）の活性を調節するカルシウム感知タンパク質である。
  • 通常、CaMによるNOSの活性化には、CaMに結合した4つのカルシウムイオンが必要である。
  • 細胞環境は分子集合を特徴とし、タンパク質のダイナミクスと機能に影響を与える。

研究 の 目的:

  • CaMのダイナミクス、CaM-酵素複合体の形成、およびCaM活性化酵素経路に対する分子集合の影響を調査すること。
  • カルシウムイオンが存在しない状態でのCaM-eNOS複合体形成を分析すること。

主な方法:

  • 一分子蛍光共鳴エネルギー移動（smFRET）分析を使用した。
  • 細胞条件をシミュレートするために、フィコール70を高分子集合体として使用した。
  • CaCl2の不存在下でCaM-eNOS複合体形成を調べた。

主要な成果:

  • 高分子はタンパク質の会合、解離、折り畳み、折り畳み解除に大きな影響を与える。
  • 分子集合は、化学的経路とは独立して、活性CaM-eNOS複合体の形成において重要な役割を果たす。
  • CaM-eNOS複合体の形成とそれに続くNO生合成は、化学的経路と力誘起経路の両方によって影響される。

結論:

  • 高分子集合は、CaM-eNOS複合体形成とNOS酵素活性の重要な調節因子である。
  • タンパク質シグナル伝達と機能は、細胞内の高分子相互作用によって調節される。
  • NOS酵素反応は、化学的および力誘起経路の組み合わせによって調節でき、新たな治療標的を提供する可能性がある。