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関連する概念動画

Defenses Against Pathogens and Herbivores02:26

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The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
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In an open-loop system, such as a basic thermostat, the poles of the transfer function influence the system's response but do not determine its stability. However, when feedback is introduced to form a closed-loop system, such as an advanced thermostat that adjusts heating based on room temperature, stability is governed by the new poles of the closed-loop transfer function.
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It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
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関連する実験動画

Updated: Feb 13, 2026

In Vitro Analysis of E3 Ubiquitin Ligase Function
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グルジア米E3リガーゼと病原体エフェクターを用いた病害防除

Yongqi Chang1,2, Lili Zhou1,2, Tongtong Zhu1,2

  • 1State Key Laboratory of Maize Bio-breeding, China Agricultural University, Beijing, 100193, China.

Journal of integrative plant biology
|February 11, 2026
PubMed
まとめ

新規化合物B93は、米のE3リガーゼAPIP6による米のブラストエフェクターAVR-PikCの分解を誘発する。この相互作用は、米のブラスト菌に対する植物抵抗性を高める。

キーワード:
米病害防除E3リガーゼ病原体エフェクターAVR-PikCAPIP6B93植物抵抗性分子メカニズム小分子

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Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
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Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
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Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
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科学分野:

  • 植物病理学
  • 分子生物学
  • 生化学

背景:

  • 米のブラスト菌（Magnaporthe oryzae）は、世界の米生産にとって重大な脅威となっている。
  • AVR-PikCなどの真菌エフェクターは、宿主プロセスを操作する主要な病原性因子である。
  • AVR-PikCは米タンパク質と相互作用し、病気の感受性に寄与する可能性がある。

研究 の 目的:

  • AVR-PikCの米における機能の分子メカニズムを調査する。
  • 米のブラスト菌に対する米抵抗性を強化する戦略を特定する。
  • 植物-病原体相互作用を調節する上での小分子の可能性を探る。

主な方法:

  • タンパク質間相互作用を検出するための酵母3ハイブリッドアッセイ。
  • 植物内での相互作用を確認するための共免疫沈降。
  • タンパク質修飾を評価するためのユビキチン化アッセイ。
  • タンパク質ターンオーバーを監視するための分解アッセイ。
  • B93で処理した米植物の表現型解析。

主要な成果:

  • 米タンパク質HIPP19がAVR-PikCの結合パートナーとして同定され、感受性媒介の可能性がある。
  • 化合物B93がAVR-PikCと米のE3リガーゼAPIP6との相互作用を誘発することがわかった。
  • この誘発された相互作用は、AVR-PikCのユビキチン化とその後の分解につながった。
  • AVR-PikCの分解により、米のブラスト菌に対する米植物の抵抗性が強化された。

結論:

  • AVR-PikC-HIPP19相互作用は、米の感受性を調節するための潜在的な標的である。
  • 小分子B93は、APIP6 E3リガーゼを介してAVR-PikCを効果的に分解標的とする。
  • 本研究は、ブラスト病原体に対する米の耐久性抵抗性を強化する新規戦略を提示する。