2つのネイティブ遺伝子を発現するゲノム編集されたトウモロコシは,北部のトウモロコシの葉の炎症に対する幅広いスペクトルの耐性を保ちます
Huirong Gao1, Bailin Li1, Kevin Fengler1
1Research and Development, Corteva Agriscience, Johnston, Iowa, USA.
Molecular plant pathology
|February 12, 2026
まとめ
ゲノム編集は,北部のトウモロコシの葉の炎症に対する耐性遺伝子をトウモロコシに正確に導入し,収穫の損失なしに作物の回復力を高めます. この技術により,広範囲の耐病性を有する商用ハイブリッドが急速に開発されています.
科学分野:
- 植物遺伝学 植物遺伝学
- 農業バイオテクノロジー 農業バイオテクノロジー
- 農作物の改良を目的とした.
背景:
- 北部のトウモロコシ葉病 (NCLB) は,トウモロコシの収穫量の損失を最大50%まで引き起こします.
- NCLB耐性に対する従来の育種は遅いもので,望ましくない特性を導入する可能性があります.
- ゲノム編集は,結合引きずりなしに抵抗性遺伝子を導入するための正確な方法を提供します.
研究 の 目的:
- CRISPR-Cas9ゲノム編集を用いてNCLB耐性トウモロコシのハイブリッドを開発する.
- エリートトウモロコシの近親種に新しい耐性遺伝子を正確に統合する.
- ゲノム編集による耐性特性の有効性と産出効果を評価する.
主な方法:
- 特定されたNCLB耐性遺伝子NLB18-Rは,Htn1およびHt2/Ht3.3へのアレルである.
- エリートトウモロコシの感受性アレルNLB18-SをNLB18-Rに置き換えるためにCRISPR-Cas9を利用した.
- リンクされたサイトにNLB18-RとHt1-Rを挿入し,遺伝子クロスで組み合わせました.
主要な成果:
- ゲノム編集されたトウモロコシの株は,NCLB (Setosphaeria turcica races 0, 1, 23N) に対する耐性を高めました.
- 編集されたラインのフィールド試験では,コントロールと比較して,有意な収量差は見られなかった.
- トウモロコシのNLB18-RとHt1-R耐性遺伝子を成功裏に積み重ねました.
結論:
- CRISPR-Cas9によるゲノム編集により,広範囲のNCLB耐性を持つトウモロコシの急速な発達が可能になりました.
- このアプローチは,従来型の育種に伴うリンクレスラグを回避します.
- ゲノム編集は,商用グレードの耐病性トウモロコシのハイブリッドを作成するための有効なツールです.
キーワード:
CRISPRは,CRISPRを活用して研究しています.HDRは,HDRで表示される.NCLBは,NCLBは,NCLBは,NCLBは,NCLBは,NCLBは,NCLBは,NCLBは,NCLBは,NCLBはアレル・スワップ・スワップとはシスゲンシスゲンシスゲン遺伝子スタック 遺伝子スタックさらに関連する動画
07:42Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing
Published on: April 27, 2018
7.9K
07:27Microinjection of Corn Planthopper, Peregrinus maidis, Embryos for CRISPR/Cas9 Genome Editing
Published on: March 26, 2021
3.1K
関連する概念動画
What is Gene Expression?
197.1K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
197.1K
What is Gene Expression?
11.6K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
11.6K
Genome Size and the Evolution of New Genes
9.2K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.2K
CRISPR/Cas9 Genome Editing
1.9K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.9K
Cell Specific Gene Expression
16.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
Chromatin Position Affects Gene Expression
24.9K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.9K
