循環する lncRNAs コメント 発現プロファイル 脳の血管変形 内皮細胞
Domenico Mordà1,2, Luigi Donato2,3, Simona Alibrandi2,3
1Department of Veterinary Sciences, Polo Universitario dell'Annunziata, Messina, Italy.
まとめ
この研究では,脳動脈静脈性変形 (bAVM) と脳洞穴性変形 (CCM) に関わる特定の長い非コーディングRNA (lncRNA) を特定しています. これらのlncRNAsは,これらの脳血管疾患のための新しい診断および治療標的を提供することができます.
科学分野:
- 血管生物学と遺伝学について
- 分子腫瘍学 分子腫瘍学
- 神経科学は神経科学である.
背景:
- 脳動脈静脈変形 (bAVM) と脳洞変形 (CCM) は,マイクロ血管に影響する脳血管変形です.
- 彼らの明確な起源には,内皮分化障害 (bAVM) と異常血管新生 (CCM) が含まれる.
- 競争性の高い内生性RNA (ceRNA) ネットワークにおけるロングノンコーディングRNA (lncRNA) の役割は,様々な疾患に関与しているが,脳血管現象型では十分に研究されていない.
研究 の 目的:
- bAVMとCCMの患者の内皮細胞 (ECs) で異なる発現のlncRNAを特定する.
- bAVMとCCMの病原性におけるこれらのlncRNAの潜在的な役割を調査する.
- lncRNAsをbAVMとCCMの潜在的な診断マーカーおよび治療標的として調査する.
主な方法:
- RNAシーケンシング (RNA-seq) とエンリッチメント分析は,bAVMとCCMバイオプシからのECで実施されました.
- 定量的リアルタイムPCR (qRT-PCR) は,ECおよび患者および対照群の血液サンプルにおけるlncRNA発現を検証するために使用されました.
- 生物情報分析により,標的のmiRNAsと,特定されたlncRNAsと関連した濃縮経路が特定されました.
主要な成果:
- 5つのlncRNA (MIR497HG,RMST,SNHG6,SOX2-OT,ZSCAN16-AS1) は,bAVM ECおよび血液で過剰発現していた.
- FGD5-AS1とSNHG12はアップレギュレーションを受け,XIST,NORAD,SNHG29,LINC0066はCCM ECでダウンレギュレーションを受けていました.
- bAVMの強化された経路には,動脈静脈分化と滑らかな筋肉細胞機能が含まれていました;CCMでは,Rho-GTPase,Delta-Notch,VEGFのシグナル伝達が顕著でした.
結論:
- 特定のlncRNAはbAVMとCCMで異なった発現を示しており,異なる病原遺伝的メカニズムへの関与を示唆しています.
- これらのlncRNAは,ceRNAとして機能し,それぞれの状態に関連するmiRNA標的を調節する可能性があります.
- この発見は,bAVMとCCMの病原性,診断,治療を調査するための新しい分野を強調しています.
キーワード:
脳の動脈静脈性異常形成である.脳の洞穴性形 (Cerebral cavernous malformation) は,脳の洞穴性形 (Cerebral cavernous malformation) と呼ばれているもので,脳内にある洞穴性形 (Cerebral cavernous malformation) は,脳の洞穴性形 (Cerebral cavernous malformation) と呼ばれている.内皮細胞 (endothelial cells) とは,内皮の細胞を構成する細胞です.lncRNAは,インクRNAに含まれている.ターゲットを絞った治療法です.さらに関連する動画
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