細胞骨格のプレストレスは,RIG-I媒介の先天免疫を調節する
Arpan Roy1, Sydney Sarver1, Jarod Beights2,3
1School of Mechanical, Aerospace, and Materials Engineering, Southern Illinois University Carbondale, Carbondale, IL 62901, USA.
まとめ
細胞の硬さが先天的免疫を調節する. 軟表面の細胞は,RIG-Iシグナル伝達に影響を与える細胞骨格前ストレスにより,抗ウイルス反応が低下し,硬い表面の細胞は,RIG-Iシグナル伝達を強化する.
科学分野:
- 免疫学 免疫学とは
- 細胞生物学 細胞生物学
- バイオフィジックス 生物物理学
背景:
- 生まれつきの免疫は,レチノ酸誘導性遺伝子I (RIG-I) のようなシグナル伝達経路を通じて重要な抗ウイルス防御を提供します.
- 細胞メカニカトランスデュークションの重要な側面である細胞骨格前ストレスのRIG-I信号伝達と先天性免疫への影響は十分に理解されていません.
研究 の 目的:
- RIG-Iシグナル伝達およびタイプIインターフェロン (IFN) 遺伝子発現の調節における細胞骨格前ストレス (cytoskeletal prestress) の役割を調査する.
- 基板の硬さが先天的な免疫反応にどのように影響するかを判断する.
主な方法:
- 細胞のトラクションとプレストレスを変化させるため,細胞を柔らかい (0.6 kPa) と硬い (8.5 kPa) 基板で培養した.
- ウイルスのdsRNAミミックであるPoly ((I:C) は,RIG-I経路を刺激するために使用されました.
- IFN-β1およびIFN刺激遺伝子 (ISG) の発現を測定した.
- TANK結合キナーゼ1 (TBK1) とYES関連タンパク質 (YAP) の活性化と局所化を評価した.
主要な成果:
- 柔らかい基板の細胞は,RIG-Iシグナリングの活性化が最小で,IFN-β1とISGの発現が低かった.
- 柔らかい基板では,YAPは細胞質に局所化し,TBK1の活性化を阻害する.
- 硬い基板の細胞は,RIG-Iシグナル伝達が強化され,TBK1の活性化が上昇し,核YAPの局所化によりIFNとISGの発現が増加した.
結論:
- 細胞骨格のプレストレスは,RIG-Iシグナリングの活性化と,その後の先天性免疫応答に大きな影響を与えます.
- 基板の硬さは,細胞骨格のプレストレスを調節し,YAPの局所化によって,抗ウイルス信号の重要な生体物理的調節体として作用します.
関連する概念動画
Introduction to Innate and Adaptive Immunity
9.9K
The human immune system is a complex defense mechanism that protects the body from harmful pathogens and foreign substances. It comprises two crucial components: innate and adaptive immunity.
Innate immunity is the body's natural, nonspecific defense system that acts quickly to protect against pathogens. It incorporates physical barriers like skin and mucous membranes and cellular elements such as phagocytes and natural killer cells. This part of our immune system provides an immediate,...
Innate immunity is the body's natural, nonspecific defense system that acts quickly to protect against pathogens. It incorporates physical barriers like skin and mucous membranes and cellular elements such as phagocytes and natural killer cells. This part of our immune system provides an immediate,...
9.9K
Cells of the Innate Immune Response
9.5K
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
9.5K
Prestressed Concrete
969
Prestressed concrete is a construction technique designed to enhance the strength and durability of concrete structures. This method involves the application of a pre-set tension to high-strength steel strands used as reinforcement before the concrete is subjected to its working loads. The primary aim of prestressing is to place the concrete in a state of compression, in order to counteract the tensile forces it will experience in service. This pre-compression helps prevent crack formation in...
969
What is the Immune System?
132.4K
Overview
132.4K
Cell-mediated Immune Responses
84.3K
Overview
84.3K
Cytotoxic T Cells-mediated Immune Response
7.6K
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
7.6K


