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Updated: Feb 13, 2026

GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins
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機能的なCas9タンパク質の効率的な浄化のための1段階の方法

Xinzhi Duan1,2, Zhengrong Zhou2, Aihua Mao1,2

  • 1Biology Department, College of Sciences, Shantou University, Shantou, China.

Bio-protocol
|February 12, 2026
PubMed
まとめ

私たちは,E. coliのユビキチン融合システムを用いて,簡素化された1段階のCRISPR/Cas9浄化方法を開発しました. このアプローチにより,ゲノム編集アプリケーション用の高純度,機能的なCas9タンパク質が得られます.

キーワード:
アフィニティクロマトグラフィーです.遺伝子エディティングプロテイン浄化 タンパク質の浄化SpCas9は9種類に分類されています.ウビキチン-融合

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