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関連する概念動画

Histone Modification02:32

Histone Modification

16.3K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
16.3K
Histone Modification02:32

Histone Modification

4.6K
4.6K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.5K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.5K
Flow Cytometry01:23

Flow Cytometry

16.5K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
16.5K
Post-translational Translocation of Proteins to the RER01:27

Post-translational Translocation of Proteins to the RER

7.8K
A sizable fraction of proteins destined for ER are first synthesized in the cell cytosol and then transported across the ER membrane–a process called post-translational translocation. Similar to cotranslationally translocated proteins, these proteins also use the Sec translocon complex to enter the ER lumen.
Targeting proteins to the ER
Hsp40 and Hsp70 chaperone molecules bind the translated proteins in the cytosol to prevent their folding. The chaperone binding helps to keep the signal...
7.8K
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

5.1K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
5.1K

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関連する実験動画

Updated: Feb 13, 2026

Author Spotlight: Enhancements in Gene Expression Regulation Research
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核ヒストン3の翻訳後の改変プロファイリングは,スペクトルフローサイトメトリーを用いて,細胞全体にプロファイリングを行います.

Carly S Golden, Saylor Williams, Sandeep Sreerama

    bioRxiv : the preprint server for biology
    |February 12, 2026
    PubMed
    まとめ
    この要約は機械生成です。

    EpiFlowは,スペクトル・フロー・サイトメトリーを用いて,全細胞におけるヒストンの翻訳後の改変 (PTMs) の多パラメータ分析を可能にします. この方法は,スループットを向上させ,クロマチンのダイナミクスの包括的な理解のためにサンプルの完全性を保ちます.

    さらに関連する動画

    Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models
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    Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models

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    Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
    11:02

    Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

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    関連する実験動画

    Last Updated: Feb 13, 2026

    Author Spotlight: Enhancements in Gene Expression Regulation Research
    07:10

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    Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models
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    Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
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    Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

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    科学分野:

    • 分子生物学は分子生物学である.
    • 細胞生物学 細胞生物学
    • エピジェネティクス エピジェネティクス

    背景:

    • ヒストンの改変は遺伝子発現と細胞のアイデンティティを調節する.
    • 多様なヒストンの翻訳後の修正 (PTM) を孤立的に研究することは,それらの協調した役割の理解を制限する.
    • ヒストンPTMのための従来のフローサイトメトリは,マルチプレキシング能力が低く,核隔離を必要とし,サンプル損失を引き起こす.

    研究 の 目的:

    • 全細胞におけるヒストンPTMの多パラメータ分析のための新しいスペクトルフローサイトメトリプロトコル,EpiFlowを開発する.
    • 従来の方法と比較して,スループット,効率,サンプルの完全性を改善します.
    • クロマチンのダイナミクスを研究するためのアクセシブルで再現可能な方法を提供すること.

    主な方法:

    • EpiFlowを開発し,全細胞ヒストンのPTM分析のためのスペクトルフローサイトメトリプロトコルである.
    • 処理能力を高めるために96井戸のプレートフォーマットを使用しました.
    • 分析プロセスを通して細胞の完全性を保ちます.

    主要な成果:

    • EpiFlowは,神経原始細胞内のヒストンPTMの微妙な変動を成功裏に解決しました.
    • この方法は,細胞サイクル全体で異なるクロマチンの状態を捉えました.
    • 結果は,いくつかの細胞マーカーと相関し,包括的な細胞状態分析を示しました.

    結論:

    • EpiFlowは,クロマチンのダイナミクスとヒストンのPTMを調査するための堅牢な枠組みを提供します.
    • このプロトコルは,多パラメータ分析を通じて,細胞のアイデンティティと機能の理解を高める.
    • オープンアクセスのリソースは,基本的および治療的研究における再現性と広範な適用性を保証します.