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関連する概念動画

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

18.7K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.7K
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

4.7K
4.7K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

10.0K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.0K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.7K
3.7K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

7.7K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.7K
Light Acquisition02:16

Light Acquisition

9.6K
In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
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関連する実験動画

Updated: Feb 13, 2026

Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran
07:35

Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran

Published on: April 28, 2011

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LUCas:光分解性干渉誘導RNAを用いた光から解放されたCas13a.

Carlos F Ng, Deepak Krishnamurthy, Andres Dextre

    bioRxiv : the preprint server for biology
    |February 12, 2026
    PubMed
    まとめ

    新しい光活性化CRISPR診断システムであるLight-Uncaged Cas13a (LUCas) は,Cas13a酵素の活性性を正確に制御しています. このイノベーションにより,感染症の検出の感度が向上し,マルチプレックスウイルス共感染の分析が可能になります.

    科学分野:

    • 分子生物学は分子生物学である.
    • バイオテクノロジー バイオテクノロジー
    • 診断用アッセイ

    背景:

    • CRISPR診断,特にCas13aを用いたCRISPR診断は,付随的な割れ目を介して,敏感で増幅のないRNA検出を提供します.
    • 制限には,背景酵素活性と反応開始の変動性が含まれ,測定の感度と解釈を妨げます.
    • これらの課題を克服するために,Cas13aの制御可能な"スタート"メカニズムが必要です.

    研究 の 目的:

    • 精密なCas13a活性化のための光制御システムを開発し,CRISPR診断の限界に対処する.
    • 分析の感度,解釈しやすさを高め,Cas13aベースの検出のための設計の可能性を拡大します.
    • 光ゲートCas13aを用いた新しいマルチプレックス検出戦略を実証する.

    主な方法:

    • 光解禁Cas13a (LUCas) の導入,光分裂性干渉ガイドRNA (pc-igRNA) を使用して,Cas13aのゲート活性.
    • 紫外線照明により,PC-igRNA抑制を解放し,Cas13aのトランス・クリバージング活動を回復します.
    • 定量運動分析,検出限界の予測と検証,およびマルチプレックス検出のためのタイムラルバーコードの実証.

    主要な成果:

    • LUCasは,光活性化の前に,Cas13aのトランス・クリアージ・アクティビティを約100倍抑制することを示しています.

    さらに関連する動画

    Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
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    Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
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    関連する実験動画

    Last Updated: Feb 13, 2026

    Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran
    07:35

    Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran

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    Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
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    Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

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    Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
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    Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

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  • このシステムは,ターゲットとは無関係なバックグラウンド活動を効果的に抑制し,予測感度決定を可能にします.
  • 実験的検証により,予測された検出限界が確認され,一時的なバーコードにより,複数のウイルス共感染の検出が可能になりました.

    • 結論:

    • LUCasは,診断アプリケーションにおけるCas13a活動の光ベースの,機械的に情報に基づいた制御のための堅牢な枠組みを提供します.
    • このシステムは,CRISPRベースの診断における感度と解釈性を大幅に改善します.
    • LUCasは,高度な複数の検出戦略を可能にし,複雑な生物サンプルに対するCRISPR診断の有用性を拡大します.