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Updated: Feb 13, 2026

Engineering Cell-permeable Protein
21:08

Engineering Cell-permeable Protein

Published on: December 28, 2009

15.0K

哺乳類の細胞における膜タンパク質-タンパク質の相互作用を,スプリットナノルシフェラーゼ経由で測定および設計するためのMemPPIプラットフォーム

Weiyi Tang1, Sebastian Jojoa-Cruz1, Jiayi Li1

  • 1Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA.

Protein science : a publication of the Protein Society
|February 12, 2026
PubMed
まとめ

この研究は,ヒト細胞における膜タンパク質-タンパク質相互作用 (memPPI) を測定するための新しいNanoBitアッセイを提示しています. 改良されたシステムは,膜タンパク質設計と薬物発見のための堅牢な定量化と高通量スクリーニングを提供します.

キーワード:
化学生物学 化学生物学高通量スクリーニング膜タンパク質は膜タンパク質である.タンパク質のデザインプロテイン工学 プロテイン工学とはタンパク質タンパク質の相互作用

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Introduction to Membrane Proteins

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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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