綿の繊維におけるGhXI-KとRab GTPasesの相互作用に関する洞察
Xinyu Li1,2, Bingke Hao1, Junwen Li1
1State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455099, China.
Plants (Basel, Switzerland)
|February 13, 2026
まとめ
ミオシンXI-Kは,綿繊維のRab GTPasesと相互作用し,モーターとテールドメインの両方を利用します. この相互作用は,膀の密輸と綿の繊維細胞の極化成長に不可欠です.
科学分野:
- 植物細胞生物学 植物細胞生物学
- 分子植物科学 分子植物科学
- 綿繊維開発について
背景:
- ミオシンXI-Kは,細胞の膨張と分極化された成長に不可欠な運動タンパク質です.
- 植物細胞における臓器細胞の密輸と細胞プラズマの流れを駆動する.
- 綿繊維の発達におけるその役割を理解することは,作物の収穫量を向上させるための鍵です.
研究 の 目的:
- 綿繊維の極化成長におけるミオシンXI-Kの分子メカニズムを調査する.
- GhXI-K (綿のミオシンXI-K) とRab GTPasesの相互作用を解明する.
- GhXI-K機能に関与する特定の相互作用サイトとドメインを特定します.
主な方法:
- 相互作用を予測するためにAlphaFold3を用いたタンパク質ドッキング分析.
- GhXI-KとRab GTPasesにおける相互作用残留物の特定.
- イーストの2ハイブリッドおよび双分子光補充 (BiFC) アッセイを用いた実験的検証.
主要な成果:
- AlphaFold3は,GhXI-Kと8つのRab GTPasesの間の相互作用を予測しました.
- GhXI-Kで37の相互作用残留物を特定し,球状尾領域 (GTD) と運動領域のキーサイトを特定した.
- 酵母2ハイブリッドとBiFCアッセイでは,主にGTDとモータードメインを含む相互作用が確認されました.
結論:
- GhXI-Kは,RabのGTPasesと,そのモーター・ドメインとテール・ドメインを通じて相互作用する.
- これらの相互作用は,分極化された膀の密輸のための相乗効果メカニズムを示唆しています.
- この発見は,綿繊維の細胞の拡大と成長の調節に関する洞察を提供します.
関連する概念動画
GTPases and their Regulation
9.9K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒ small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
Large G-proteins,...
9.9K
GTPases and their Regulation
3.0K
3.0K
Rab Cascades
3.6K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
3.6K
Rab Proteins
5.2K
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
5.2K
Coat Assembly and GTPases
4.5K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
4.5K
Small GTPases - Ras and Rho
5.5K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
5.5K


