Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Cis-regulatory Sequences02:02

Cis-regulatory Sequences

11.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.9K
Sequences01:29

Sequences

296
Sequences are fundamental mathematical objects consisting of ordered lists of numbers that follow a specific rule or pattern. Sequences are critical in various mathematical concepts, including calculus, series, and number theory. They can model real-world phenomena such as population growth, financial investments, and physical processes like the diminishing height of a bouncing ball.Each number in a sequence is referred to as a term. Typically, the terms are denoted as a1, a2, a3,…, where...
296
Sanger Sequencing01:57

Sanger Sequencing

775.1K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
775.1K
Arithmetic Sequences01:30

Arithmetic Sequences

243
An arithmetic sequence is a structured arrangement of numbers where each term is derived by adding a constant value, known as the common difference, to the previous term. This consistent pattern allows for the efficient computation of any term within the sequence as well as the cumulative sum of multiple terms. The formula for finding the nth term of an arithmetic sequence is:Here, aₙ represents the nth term of the sequence, a is the first term, d is the common difference, and n is the...
243
Geometric Sequences01:30

Geometric Sequences

294
In systems where values diminish by a constant proportion at each stage, the resulting sequence follows a geometric structure. Each new value in the sequence is obtained by applying a fixed multiplier to the preceding term. This regular, proportional decline type is often used to represent processes involving gradual loss, such as energy dissipation or reduction in amplitude over time.When analyzing the total effect of such a process across unlimited iterations, the series of values is referred...
294
Next-generation Sequencing03:00

Next-generation Sequencing

98.9K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
98.9K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Genetic Analysis Reveals Climate Exposure and Conservation Opportunities for a Vulnerable Life History in Steelhead (<i>Oncorhynchus mykiss</i>).

Evolutionary applications·2026
Same author

Short-Term Outcomes in Neonates Born to Diabetic Mothers During the Coronavirus Disease 2019 Pandemic: A Descriptive Study.

AJP reports·2026
Same author

Post-cardiac arrest care during transport from referral centers to a tertiary children's hospital.

Resuscitation plus·2026
Same author

Evaluating intramuscular epinephrine in pediatric out-of-hospital cardiac arrest (the PRIME trial): study protocol for a multi-centre, stepped-wedge cluster quasi-randomized controlled trial.

Trials·2026
Same author

The Vigilance Gradient: Nine Years of Adverse Event Trends in Pediatric Critical Care.

Critical care explorations·2026
Same author

Management of serious <i>Staphylococcus aureus</i> infections in children: A survey of paediatric infectious diseases physicians in Canada.

Paediatrics & child health·2026

関連する実験動画

Updated: Feb 14, 2026

Amplicon Sequencing using the Long-Read Sequencing Technologies
08:57

Amplicon Sequencing using the Long-Read Sequencing Technologies

Published on: August 29, 2025

551

AmpliRAD:アンプリコンとRADシーケンシングを組み合わせた新しい方法

Tasha Q Thompson1, Michael R Miller2, Matthew R Sloat1

  • 1Wild Salmon Center Portland Oregon USA.

Ecology and evolution
|February 13, 2026
PubMed
まとめ

新しい方法であるampliRADは,全ゲノムSNPデータのための標的と減少表現配列を組み合わせています. このアプローチにより,GREB1Lの場所とチヌーク・サーモンの移動のタイミングを成功裏に結びつけました.

科学分野:

  • ゲノミクスゲノミクスとは
  • 分子生物学は分子生物学である.
  • 人口遺伝学 人口遺伝学

背景:

  • RAD-seqのような減少表現シーケンシング方法は,ゲノム全体のSNPデータに対して費用対効果的です.
  • RAD-seqの重要な制限は,制限部位の近くにない特定の場所をターゲットにすることができないことです.

研究 の 目的:

  • ターゲティング・シーケンシングとリデュースされた表現シーケンシングを統合する新しい方法であるampliRADを導入する.
  • RADシーケンシングプロトコルのアクセシビリティと効率を高めるために.
  • チヌーク・サーモンの移住タイミングと遺伝的関連性を調査する.

主な方法:

  • AmpliRADはマルチプレックスPCRを使用して,ターゲットロキーを増幅し,制限部位を付加します.
  • 増幅されたターゲットは,標準的なRADライブラリ準備のためにゲノムDNAと組み合わせられます.
  • 酵素切断とプロトコルアップデートにより,RADシーケンシングの効率が向上します.

主要な成果:

  • AmpliRADは,RADのデータセットに実質的に任意のターゲットロキを成功裏に組み込みます.
  • GREB1Lの位置と移住のタイミングの間の遺伝的関連は,チヌーク・サーモンで特定されました.

さらに関連する動画

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
07:21

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing

Published on: August 25, 2018

13.5K
Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

20.7K

関連する実験動画

Last Updated: Feb 14, 2026

Amplicon Sequencing using the Long-Read Sequencing Technologies
08:57

Amplicon Sequencing using the Long-Read Sequencing Technologies

Published on: August 29, 2025

551
Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
07:21

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing

Published on: August 25, 2018

13.5K
Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

20.7K
  • 発見は,以前の移住のタイミング関連を北部の河川の集団に拡張します.

    • 結論:

    • AmpliRADは,ゲノム分析のための強力なツールを提供し,減少表現と標的配列化の利点を組み合わせています.
    • この研究は,移住のタイミングのような複雑な特徴の遺伝的基盤を明らかにする上でampliRADの有用性を強調しています.
    • この方法は,ゲノム全体の文脈で特定の位置を研究する能力を向上させます.