潜在的異性型窒素化-エアロビック脱窒化およびリンを蓄積する細菌Pseudomonas putida S3およびその栄養素除去性能の分離と特徴付け
Yifan Zhang1, Yating Sun1, Yuwei Xu1
1Anhui Province Key Laboratory of Pollution Damage and Biological Control for Huaihe River Basin, College of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China.
まとめ
新種の細菌,Pseudomonas putida S3は,低炭素から窒素に変換された廃水から窒素とリンを効率的に除去します. この微生物は炭素を効果的に利用し,排水処理の課題に有望な解決策を提供している.
科学分野:
- 環境微生物学 環境微生物学
- 排水処理技術について
- バイオリメディエーションとは
背景:
- 排水中の低炭素/窒素 (C/N) 比は,効率的な窒素とリン除去を制限する.
- ヘテロトロフィックな窒素化-エアロビックな脱窒素化とリンの蓄積を行う選択的な微生物菌株は,栄養素の除去を強化するために不可欠です.
- 単一の生物学的プロセス内の炭素利用を最大化することは,排水処理効率を改善する鍵です.
研究 の 目的:
- 低C/Nの廃水から同時に窒素とリンを除去する多機能細菌を分離し,特徴づけること.
- Pseudomonas putida S3.を使用した栄養素除去条件を最適化するために.
- 先進的な排水処理と資源回収のためのPseudomonas putida S3の可能性を評価する.
主な方法:
- 都市排水処理所の泥から多機能細菌,Pseudomonas putida S3を分離した.
- 特定の炭素と窒素の源,C/NとN/P比を用いた栄養素除去のためのバッチ実験の最適化.
- 菌株S3のエアロビック栽培で,同時に窒素とリンを除去する能力を評価する.
主要な成果:
- Pseudomonas putida S3は,エアロビック条件下で48時間以内に,アンモニア窒素 (73.33%) と総窒素 (71.53%) を効率的に同時に除去することを実証しました.
- 菌株S3はエアロビックによる水相からの完全なリン除去を達成し,細胞内メタクロマティック粒子を形成しました.
- 最適な条件には,炭素源としてナトリウムシトラート,窒素源としてアンモニアム塩化物,C/N比6およびN/P比15が含まれる.
結論:
- Pseudomonas putida S3は,低C/Nの廃水の高度な処理のための有望な微生物候補である.
- この研究は,多機能バクテリアによる余剰スラグの資源指向的な利用のための理論的基礎を提供します.
- 特定された株は,単一の生物学的プロセスで炭素の利用を最大化することによって,栄養素の除去効率を高めます.
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