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DNA Topoisomerases02:02

DNA Topoisomerases

35.9K
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
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Complementary DNA01:44

Complementary DNA

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Overview
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DNA Replication02:40

DNA Replication

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DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
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Bacterial Transformation01:33

Bacterial Transformation

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In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
60.2K
DNA-only Transposons02:57

DNA-only Transposons

17.6K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
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Recombinant DNA01:09

Recombinant DNA

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Overview
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Updated: Feb 15, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

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微生物プライマー:バクテリアDNAのスーパーコーリング

Charles J Dorman1

  • 1Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College Dublin, Dublin D02 PN40, Ireland.

Microbiology (Reading, England)
|February 13, 2026
PubMed
まとめ
この要約は機械生成です。

超回転と鎖分離によって維持される細菌のDNAトポロジーは,トポイソメラーゼ酵素によって管理されます. DNAトポロジーの理解は,細菌の病原性および転写と複製のような細胞プロセスにとって非常に重要です.

キーワード:
DNAスーパーコイリングDNAトポイソメラゼは,DNAトポイソメラゼをトランスクリプション トランスクリプションツイン・スーパーコーリング・ドメイン・モデル

さらに関連する動画

Extraction of High Molecular Weight DNA from Microbial Mats
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Extraction of High Molecular Weight DNA from Microbial Mats

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Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
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Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

Published on: September 3, 2009

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関連する実験動画

Last Updated: Feb 15, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

25.4K
Extraction of High Molecular Weight DNA from Microbial Mats
09:30

Extraction of High Molecular Weight DNA from Microbial Mats

Published on: July 7, 2011

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Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
15:28

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

Published on: September 3, 2009

20.8K

科学分野:

  • 微生物学 微生物学とは
  • 分子生物学は分子生物学である.
  • バイオケミストリー バイオケミストリー

背景:

  • バクテリアのDNAは,アンダーウォンド状態で存在します.
  • DNAは,スーパーコイリングまたは単一鎖の泡形成を通じて,アンダーウィンドリングに反応します.
  • DNAトポロジーは,細胞機能に大きく影響する.

研究 の 目的:

  • バクテリアの重要なDNAトポロジカル特徴を要約する.
  • DNAトポロジーの管理に関与するトポイソメラーゼ酵素を記述する.
  • DNAトポロジー,転写,複製,代謝,および病原性との相互作用について議論する.

主な方法:

  • 文献レビューと既存の研究の合成.
  • DNAスーパーコイリングとトポイソメラーゼ機能の説明.
  • 遺伝子発現とDNA複製に対するトポロジカルな影響に関する議論.

主要な成果:

  • DNAトポロジーは,細菌生理学の重要な要因である.
  • トポイソメラーゼ酵素は,DNA構造の調節に不可欠です.
  • DNAトポロジーの変化は,細菌の病原性に影響する.

結論:

  • バクテリアのDNAトポロジーは,細胞プロセスによって影響されるダイナミックな特徴です.
  • トポアイソメラーゼは,治療介入の重要な標的である.
  • DNAトポロジーの理解は,細菌の生存と毒性の洞察を提供します.