N-イソトープの最小侵襲的希釈法を使用して, brojler の全内生窒素損失の定量化
M F Habibi1, S Dorado-Montenegro2, R P Kwakkel3
1Animal Nutrition Group, Wageningen University & Research, P.O. Box 338, 6700 AH Wageningen, the Netherlands; Faculty of Animal Science, Universitas Gadjah Mada, Sleman, Yogyakarta 55281, Indonesia.
Animal : an international journal of animal bioscience
|February 13, 2026
まとめ
新しい最小侵襲的同位素稀解法 (ID) が,ブローラーにおける全内生窒素損失 (tENL) を正確に定量化しています. この方法は,尿15N濃縮を用いて,さまざまな食事の間で真の窒素消化性を評価するための実用的な代替案を提供します.
科学分野:
- 動物の栄養と代謝について
- 消化性と窒素バランスに関する研究
- イソトープトレーサーの家禽への応用
背景:
- 総内生窒素損失 (tENL) の正確な測定は,食中の真の窒素の消化率を決定するために非常に重要です.
- 酵素化水解カゼイン (EHC) やEHC-超濾過 (EHC-UF) などの現在の方法は,食事特有のtENL定量化に限界があります.
- 15N同位体稀解法 (ID) は,基礎および食事特有のENLを測定するための潜在的なアプローチを提供します.
研究 の 目的:
- 15N同位体稀解法 (ID) の最小侵襲的な方法を使用して, brojler の全内生窒素損失 (tENL) を定量化します.
- IDメソッドの推定値を従来のEHCおよびEHC-UFメソッドと比較して検証する.
- 尿15N濃縮の有用性を,前駆体プール15N濃縮の代理として,さまざまな食事を与えられたブローラーで評価する.
主な方法:
- 合計432匹の雌の brojler は,繊維の成分が異なるダイエットや,EHC.を含む半合成ダイエットで餌を与えられました.
- 15Nイソトープ溶液 (ミルクタンパク質濃縮物とアンモニアム塩化物) を26日から31日までブライラに口服した.
- 排泄物,イレアル・ダイジェスタ,および組織サンプルが窒素および15N濃縮分析のために収集され,尿15N濃縮は前駆体プールプロキシとして機能しました.
主要な成果:
- 尿15N濃縮は急速に安定状態に達し,組織15N濃縮と良好に相関する (r=0.84-0.90).
- ID方法によるIleal tENLの推定値は,EHC (-75.6%) およびEHC-UF (-70.3%) 方法によるものより著しく低かった.
- ID方法は,EHC/EHC-UFと比較して,少し低い真のイレアル窒素消化率の推定値 (-1〜-4%単位) をもたらしました.
結論:
- 侵襲性最小の15N同位体稀解法 (Minimally invasive 15N-isotope dilution method) は, brojler の全内生窒素損失を定量化するための実行可能で実用的なツールです.
- 尿中の15N濃縮を有効に利用することで,前駆体プールが形成され,さまざまな食物繊維とタンパク質の源でtENLの定量化が可能になります.
- ID方法は,全内生窒素損失を比較し,家禽における真のイレアル窒素消化性を評価するための貴重な代替手段を提供します.
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