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関連する概念動画

Confidence Interval for Estimating Population Mean01:25

Confidence Interval for Estimating Population Mean

9.0K
A point estimate of the population mean is obtained from a single sample. Such a point estimate does not represent a population well because it needs to account for variability in the population. Single point estimate can also be biased despite the sample being selected randomly. Thus, a point estimate is often unreliable. A confidence interval is needed to reduce this unreliability.
A confidence interval for the mean is a range of values that provides an estimate of the population mean. As the...
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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

9.0K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.6K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.6K
Mass Spectrometry of Amines01:15

Mass Spectrometry of Amines

5.4K
In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule; a molecule with an odd number of nitrogen atoms produces a molecular ion with an odd molecular weight. Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit strong molecular ion peaks, but acyclic...
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Mass Spectrometry: Isotope Effect01:13

Mass Spectrometry: Isotope Effect

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Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
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Chemical Equilibria: Systematic Approach to Equilibrium Calculations01:21

Chemical Equilibria: Systematic Approach to Equilibrium Calculations

1.7K
Equilibrium calculations for systems involving multiple equilibria are often complex. For example, to calculate the solubility of a sparingly soluble salt in an aqueous solution in the presence of a common ion, one must consider all the equilibria in this solution. Calculations for these systems can be complicated and tedious, so a systematic approach with a series of steps is often helpful. The process is detailed below.
The first step is to identify all the chemical reactions involved, The...
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関連する実験動画

Updated: Feb 15, 2026

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
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死後の間隔推定のための質量スペクトロメトリーベースのプロテオミックアプローチ: 体系的なレビュー

Rakshita Gautam1, Akansha Das1, Sachil Kumar1

  • 1Amity Institute of Forensic Sciences, Amity University, Noida, 201313, Uttar Pradesh, India.

Journal of forensic and legal medicine
|February 13, 2026
PubMed
まとめ

質量スペクトロメトリーベースのプロテオミクスは,タンパク質の分解を分析することによって,死後の間隔 (PMI) を推定するための敏感な方法を提供します. ルーティンの法医応用には,さらなる標準化と検証が必要である.

キーワード:
バイオマーカー バイオマーカー法医学プロテオミクスLCMS/MS/MS について質量スペクトロメトリによる質量スペクトロメトリです.死後の間隔は,死後の間隔である.タンパク質の分解 タンパク質の分解死亡以来の時間.

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Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
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Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
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Last Updated: Feb 15, 2026

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
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Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
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Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

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科学分野:

  • 法医学科学は,法医科学である.
  • バイオケミストリー バイオケミストリー
  • アナリティカル・ケミストリー (Analytical Chemistry) とは

背景:

  • 正確な死後の間隔 (PMI) の推定は,法医学調査において極めて重要です.
  • 伝統的なPMI方法は,さまざまな要因の影響を受け,信頼性を制限しています.
  • 質量スペクトロメトリ (MS) ベースのプロテオミクスは,時間依存のタンパク質分解を分析することによって,新しいアプローチを提供します.

研究 の 目的:

  • PMI推定のためのMSベースのプロテオミック技術における最近の進歩を体系的にレビューする.
  • 死後の分解を示す再現可能なタンパク質バイオマーカーを特定する.
  • これらのタンパク質学的アプローチの法医学的適用性を評価する.

主な方法:

  • 体系的な文献検索 (2020-2025) は,PubMed,Scopus,およびScienceDirect.で実施されています.
  • PMIの推定のためにMSベースのプロテオミクスを用いたオリジナルの研究を含む.
  • レビュー,非英語の記事,重複の除外;独立したデータ抽出.

主要な成果:

  • 21件の研究が含まれており (ヒト10例,動物10例).
  • 骨格筋,骨,肝臓,心臓,および歯茎は,分析された一般的な組織でした.
  • トロポミオシン,GAPDH,デスミン,チューブリン,eEF1A2などのタンパク質は,再現可能な分解パターンを示した.
  • 様々なMS技術 (GC-MS,LC-MS/MS,MALDI-MS) が採用されました.
  • プロトコルの有意な変動性により,標準化が必要となる.

結論:

  • MSベースのプロテオミクスは,PMI推定のための敏感で客観的な方法を提供します.
  • いくつかのタンパク質バイオマーカーは,潜在的な法医学的な有用性を示しています.
  • 標準化,より大きな人体検証研究,および多タンパク質パネルが,より広範な法医学的な適用のために必要とされています.