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関連する概念動画

Channel Rhodopsins01:11

Channel Rhodopsins

3.3K
Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
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Voltage01:13

Voltage

4.3K
The movement of electrons in a conductor requires some form of energy or work, usually provided by an external force, like a battery. This force is called the electromotive force or voltage. The voltage between two points, referred to as points "a" and "b," in an electric circuit is the energy (or work) needed to move a unit charge from point "a" to point "b," and this relationship is expressed mathematically as
4.3K
Multiple Voltage Sources01:25

Multiple Voltage Sources

1.8K
Generally, a single battery is not enough to power some devices. In such cases, batteries can be combined in two ways: in series or in parallel.
In series, the positive terminal of one battery is connected to the negative terminal of another battery. Hence, the voltage of each battery is added to give the net voltage, which is increased because each battery boosts the electrons that enter it. The same current flows through each battery because they are connected in series.
Batteries are...
1.8K
Voltage Dividers01:14

Voltage Dividers

1.4K
In electrical circuits, resistors can be connected in series, sequentially linked one after the other. In a series configuration, the same current flows through each resistor. Ohm's law is a fundamental principle to understand the behavior of resistors in series. It expresses the voltage across these resistors in terms of the current and resistance.
Kirchhoff's voltage law implies that the sum of the voltages across the resistors in series equals the source voltage. This means that the current...
1.4K
Three-Phase Voltages01:30

Three-Phase Voltages

611
A three-phase generator produces three voltages that are equal in magnitude but have a phase difference of 120 degrees. This identical magnitude and equal phase separated voltages are known as the balanced voltages and help to minimize power loss while ensuring a steady delivery of energy to connected loads. As voltage sources in a three-phase system can be configured in a wye or a delta formation, the loads connected to these systems can also be arranged in either configuration. This...
611
Nodal Analysis with Voltage Sources01:11

Nodal Analysis with Voltage Sources

2.0K
Nodal analysis is a remarkably effective method used in electrical engineering to simplify the analysis of complex circuits, including those with dependent or independent voltage sources. Its strength lies in its systematic approach to breaking down circuits into manageable components, making it easier for engineers to understand and solve.
Consider a circuit that contains four resistors and two voltage sources, as shown in Figure 1. One of these voltage sources is connected between a...
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関連する実験動画

Updated: Feb 15, 2026

A Rhodopsin Transport Assay by High-Content Imaging Analysis
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A Rhodopsin Transport Assay by High-Content Imaging Analysis

Published on: January 16, 2019

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ロードプシンベースのセンサーによる2フォトン電圧イメージング

Christiane Grimm1, Ruth R Sims1, Dimitrii Tanese1

  • 1Institut de la Vision, Sorbonne Université, INSERM, CNRS, 75012 Paris, France.

Neuron
|February 13, 2026
PubMed
まとめ

研究者は,2フォトン (2P) イメージングのために,ロドプシンベースの遺伝子コード化された電圧指標 (GEVI) を設計した. このブレークスルーにより,高速で,信号と騒音の比率が高いアクションポテンシャルの検出が,in vivoおよびin vitroで可能になりました.

キーワード:
アクションポテンシャル検出 検出急速な機能的画像処理が可能である.ホログラム照明によるホログラム照明微生物のロドプシンである.スキャンレス画像処理2フォトン顕微鏡による2フォトン顕微鏡電圧指示器の電圧指示器の電圧指示器の電圧指示器の電圧指示器の電圧指示器の電圧指示器の電圧指示器の電圧指示器の電圧指示器の電圧指示器の電圧指示器の電圧指示器の電圧指示器の電圧指示器

さらに関連する動画

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
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Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

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Nanostructured Ag-zeolite Composites as Luminescence-based Humidity Sensors
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Nanostructured Ag-zeolite Composites as Luminescence-based Humidity Sensors

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関連する実験動画

Last Updated: Feb 15, 2026

A Rhodopsin Transport Assay by High-Content Imaging Analysis
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A Rhodopsin Transport Assay by High-Content Imaging Analysis

Published on: January 16, 2019

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Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
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Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

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Nanostructured Ag-zeolite Composites as Luminescence-based Humidity Sensors
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Nanostructured Ag-zeolite Composites as Luminescence-based Humidity Sensors

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科学分野:

  • 神経科学は神経科学である.
  • オプティカルイマージング (光学画像)
  • 分子生物学は分子生物学である.

背景:

  • 2フォトン (2P) の電圧イメージングは,高度な解像度でニューロンの活動を徹底的にモニタリングします.
  • 現在の2P電圧イメージングは主にASAPタイプのセンサーを使用し,ロドプシンベースのセンサーは主に1フォトン (1P) 照明で使用されています.

研究 の 目的:

  • 2P照明によるロドプシンベースの遺伝的にコードされた電圧指示器 (GEVIs) の互換性を実証する.
  • 改良された2P電圧イメージングのための新しいロドプシンベースのGEVIを設計する.
  • これらのセンサーの性能を,様々な生物学的調合物や条件下で評価する.

主な方法:

  • ジャービスという新しいロドプシンベースのGEVIの合理的な工学.
  • ジャルビスと既存のFRET-オプシンGEVIs (pAce,Voltron2) を1Pと2P照明下でテストする.
  • 2Pスキャンレスと高速の2Pスキャンによる照明方法を比較する.
  • マウスのヒポキャンパスのスライス,ゼブラフィッシュの幼虫,目覚めているマウスの皮質におけるアクションポテンシャル検出の評価.

主要な成果:

  • Jarvis,pAce,Voltron2.2を含むロドプシンベースのGEVIsに対する2P照明の互換性を成功裏に実証しました.
  • スキャンレス2P照明を用いた低照射下でのFRETオプシンGEVIsの改善された信号対ノイズ比 (SNR) を特定しました.
  • JarvisとpAceを用いてキロヘルツで高SNRアクションポテンシャル検出を in vitroとin vivoで達成しました.

結論:

  • ロドプシンベースのGEVIは,高性能2P電圧イメージングのための効果的なツールです.
  • スキャンレス 2P 照明は,FRET-オプシン GEVI の SNR を強化します.
  • この研究は,複雑な生物学的システムにおける深度で高速なニューロン活動モニタリングのためのツールキットを拡張します.