Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Translesion DNA Polymerases02:10

Translesion DNA Polymerases

11.3K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
11.3K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

32.9K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
32.9K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

27.2K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
27.2K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

11.1K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
11.1K
DNA as a Genetic Template02:05

DNA as a Genetic Template

28.0K
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
28.0K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.4K
Overview
80.4K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Impact of neoadjuvant chemotherapy vs. upfront surgery on survival in early-onset colorectal cancer-a single centre experience.

Chinese clinical oncology·2026
Same author

Efficacy of lifestyle interventions in treating erectile dysfunction: a systematic review and meta-analysis of randomized controlled trials.

The journal of sexual medicine·2026
Same author

Synergistic interfaces in a MoC-Ni<sub>4</sub>Mo-Ni<sub>2</sub>P heterostructure drive durable bifunctional electrocatalysis for industrial water splitting.

Journal of colloid and interface science·2026
Same author

A Comparative Review of Veterinary and Human Vaccine Development Strategies: Insights into Herpesvirus Vaccinology from Latency to Elimination.

Vaccines·2026
Same author

Comparative efficacy and safety of non-pharmacological interventions for erectile dysfunction:a systematic review and network meta-analysis.

Sexual medicine reviews·2026
Same author

The ultra-large-capacity silica-supported ferric oxyhydroxide material: Preparation and mechanism on arsenic removal from water.

Environmental research·2026

関連する実験動画

Updated: Feb 15, 2026

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells
14:26

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Published on: April 4, 2016

26.0K

循環変異工学は,Bst DNAポリメラーゼの増幅効率を向上させます.

Rong Xiang1, GuangYi Liu2, YanRu Wang1

  • 1School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China.

International journal of biological macromolecules
|February 13, 2026
PubMed
まとめ

研究者は,強化された核酸検出のための新しいDNAポリメラーゼ (CP-G23) を設計しました. このポリメラーゼはプライマーの設計を簡素化し,ループ媒介型同熱増幅 (LAMP) アッセイにおける逆転写酵素の必要性を排除します.

キーワード:
BstDNAポリメラーゼは,BstDNAポリメラーゼと一致している.円周パルムテーションです.阻害剤に対する耐性は高い.ループ媒介型同熱増幅 (LAMP)リバーストランスクリプションLAMP (RT-LAMP)

さらに関連する動画

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.3K
Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

14.8K

関連する実験動画

Last Updated: Feb 15, 2026

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells
14:26

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Published on: April 4, 2016

26.0K
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.3K
Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

14.8K

科学分野:

  • 分子生物学は分子生物学である.
  • バイオテクノロジー バイオテクノロジー
  • 診断用アッセイ

背景:

  • 化学的なBstDNAポリメラーゼ (HpStBL) は,核酸検出の有望性を示したが,限界に直面した.
  • 複雑なプライマーの設計と非特異的な増幅は,ループ介的同熱増幅 (LAMP) で HpStBL のより広範な適用を妨げました.

研究 の 目的:

  • ループ媒介型同熱増幅 (LAMP) と逆転写LAMP (RT-LAMP) のための改良されたDNAポリメラーゼを開発する.
  • 性能が向上した,簡素化され,迅速な核酸検出方法を作成する.

主な方法:

  • HpSt.BL.のHp47-Sto7dドメインを変更することによって,四つの円周パルミュテーション (CP) のBstDNAポリメラーゼ変異体を生成しました.
  • 変異体のDNA合成と逆転写酵素の活性を評価した.
  • CP-G23変異体とThermus thermophilus recombinaseを用いた核酸検出システムを開発した.

主要な成果:

  • 円形変異はタンパク質の水嫌性とDNAの親和性を変化させ,DNA合成と逆転写酵素活性を持つ変異体を生成しました.
  • 変異したCP-G23は優れた増幅性能を示し,100コピー/μLのアストロウイルスプラズミドDNAを検出しました.
  • CP-G23ベースのシステムは,リバーストランスクリプタゼを必要とせずに,単一のプライマーペアを使用して,45分でアストロウイルスDNAとSARS-CoV-2RNAを成功裏に増幅しました.

結論:

  • LAMPアッセイにおけるDNAポリメラーゼの性能を改善するための効果的な戦略は,円周パルミュテーションです.
  • 新規のCP-G23ポリメラーゼおよび関連する検出方法は,DNA/RNA検出の簡素化,迅速化,および敏感なアプローチを提供します.
  • この方法は,臨床診断と分子検査の大きな可能性を秘めています.