周辺血液単核細胞の表面染色により,従来のおよび非従来のT細胞集団の免疫フェノタイプ化
Andrea Leufgen1, Annika Steitz1, Conrad Rauber2
1Clinical Cooperation Unit Applied Tumor Immunity, German Cancer Research Center (DKFZ), Heidelberg, Germany; National Center for Tumor Diseases (NCT) Heidelberg, A partnership between DKFZ and Heidelberg University Medical Center, Heidelberg, Germany.
Methods in cell biology
|February 13, 2026
まとめ
この研究では,先端の28色のフローサイトメトリを使用して,ヒトの周辺血液単核細胞 (PBMC) を分離,保存,分析するための堅牢なプロトコルについて詳細に述べています. この方法は,様々な臨床研究アプリケーションの免疫モニタリングを強化します.
科学分野:
- 免疫学 免疫学とは
- 細胞生物学 細胞生物学
- バイオテクノロジー バイオテクノロジー
背景:
- ヒト外周血液単核細胞 (PBMC) の正確な免疫フェノタイプ化は,トランスレーションおよび臨床免疫学にとって極めて重要です.
- 既存のプロトコルには,冷凍保存と高次元分析の標準化が欠如し,縦線または多センター研究が制限されている可能性があります.
研究 の 目的:
- PBMCの隔離,冷凍保存,28色フローサイトメトリの免疫フェノタイプ化のための包括的で再現可能なプロトコルを提示する.
- 多様なT細胞集団とその機能的状態の同時評価を可能にする.
主な方法:
- 血液処理の詳細な手順,密度グラデーション分離,血小板汚染を減らすために最適化された洗浄.
- 細胞の生存能力と機能を維持するための標準化された冷凍保存技術.
- PBMC染色に最適化された28色のフローサイトメトリーパネル,従来のスペクトルシステムと互換性があります.
主要な成果:
- このプロトコルは,リンパ球集団,特にTリンパ球の堅牢で再現可能な識別を可能にします.
- 健康なドナーおよび患者からの新鮮なおよび冷凍保存されたPBMCの両方に有効です.
- 感染症,がん,免疫療法における免疫モニタリングに適用できることを示しています.
結論:
- このプロトコルは,PBMCsの高次元免疫プロファイリングのための信頼できる枠組みを提供します.
- ワークフローは適応可能で,標準化された免疫モニタリングパイプラインに統合できます.
- 異なる研究環境と患者コホートにおける一貫した免疫細胞分析を容易にする.
キーワード:
フローサイトメトリーフローサイトメトリーPBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,PBMCsは,そしてT細胞とT細胞の違いハイ・スループット・スループット・ハイ・スループットさらに関連する動画
09:45Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
Published on: January 1, 2017
11.5K
12:52Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
Published on: November 28, 2015
16.6K
関連する概念動画
01:30Sign Convention
3.6K
When analyzing a beam subjected to various loads, it is crucial to understand the internal forces and moments generated within the structure. These internal forces can be broadly classified into normal forces, shear forces, and bending moments. To determine these forces and moments, we use the method of sections and apply a specific sign convention based on their direction and the side of the section being analyzed.
The normal force acts perpendicular to the beam's cross-section and can...
The normal force acts perpendicular to the beam's cross-section and can...
3.6K
01:15Enolate Mechanism Conventions
3.0K
When a carbonyl compound is treated with a strong base, the α position gets deprotonated to give a resonance-stabilized intermediate called an enolate. Enolates are ambident nucleophiles because they possess two nucleophilic sites that can attack an electrophile owing to the delocalization of the negative charge between the α carbon and oxygen atoms. When the oxygen atom attacks an electrophile, it is called O-attack, whereas electrophilic attack via the α carbon is known as...
3.0K
01:21Dose Response Curve: Conventional Versus Nonmonotonic
28
The correlation between a drug's dosage and its impact on a biological system is a cornerstone of pharmacology and toxicology. Conventional dose–response curves, which include graded and quantal relationships, are key to this understanding. Graded dose–response curves depict the spectrum of a biological reaction to different doses within an individual, indicating that as the drug dosage increases, so does the intensity of the response. On the other hand, quantal dose–response...
28
01:21Cell-surface Signaling
54.9K
Hormones—or any molecule that binds to a receptor, known as a ligand—that are lipid-insoluble (water-soluble) are not able to diffuse across the cell membrane. In order to be able to affect a cell without entering it, these hormones bind to receptors on the cell membrane. When a first messenger, a hormone, binds to a receptor, a signal cascade is set off, causing second messengers, proteins inside the cell, to become activated, resulting in downstream effects.
54.9K
01:28Types of Receptors: Cell Surface Receptors
28.0K
Cell-surface receptors, also known as transmembrane receptors, are cell surface, membrane-anchored (integral) proteins that bind to external ligand molecules. This type of receptor spans the plasma membrane and performs signal transduction, converting an extracellular signal into an intracellular signal. Ligands that interact with cell-surface receptors do not have to enter the cell that they affect. Cell-surface receptors are also called cell-specific proteins or markers because they are...
28.0K
01:20Protein Buffers in Blood Plasma and Cells
4.0K
The human body utilizes protein buffer systems to maintain a stable pH. These systems capitalize on the dual role of amino acids, which can act as acids or bases by accepting or releasing hydrogen ions in response to pH changes. Protein buffer systems are particularly significant in the extracellular fluid (ECF) and intracellular fluid (ICF) of active cells, where structural and functional proteins provide substantial buffering capacity.
Certain amino acids can exist in a zwitterion state at a...
Certain amino acids can exist in a zwitterion state at a...
4.0K