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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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The Proteasome02:18

The Proteasome

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Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
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The Proteasome01:13

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Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
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The Proteasome02:18

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What is Gene Expression?01:42

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Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
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Cell Type-specific Gene Expression Profiling in the Mouse Liver
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プロテアソーム活動は,細胞型特異の遺伝子発現を維持する.

Xiuxiu Lu1, Vasty Osei-Amponsa1, Germán Michelis2

  • 1Protein Processing Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA.

Cell reports
|February 14, 2026
PubMed
まとめ
この要約は機械生成です。

プロテアゾーム活動は,遺伝子発現を調節することによって,細胞の同一性を維持するために不可欠です. hRpn10/PSMD4におけるユビキチン結合の喪失は,これを破壊し,誤った発現したタンパク質と変異した細胞タイプにつながる.

キーワード:
CP:分子生物学についてOTUD5はオットゥド5です.PSMD4 PSMD4 についてRpn10 Rpn10 Rpn10 Rpn10 Rpn10 Rpn10 Rpn10 Rpn10 Rpn10 Rpn10 Rpn10 Rpn10細胞のアイデンティティ 細胞のアイデンティティ遺伝子発現は遺伝子発現であるプロテアゾームタンパク質は,タンパク質の分解 タンパク質の分解組織特異性 組織特異性トランスクリプション規制ユビキチン (Ubiquitin) とは どこにでもいる

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Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue
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Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
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Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
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科学分野:

  • 分子生物学は分子生物学である.
  • 細胞生物学 細胞生物学
  • バイオケミストリー バイオケミストリー

背景:

  • ユビキチン-プロテアゾーム系を介して制御されたタンパク質分解は,タンパク質の品質管理,細胞サイクル,DNA修復などの細胞機能に不可欠です.
  • プロテアソーム基板受容体hRpn10/PSMD4のユビキチン結合活動は,基板の認識と分解に不可欠である.

研究 の 目的:

  • 細胞同一性の維持におけるプロテアソーム活性,特にhRpn10/PSMD4のユビキチン結合機能の役割を調査する.
  • プロテアソーム機能障害が細胞型特異的な遺伝子発現に影響を与える分子機構を特定する.

主な方法:

  • ユビキチン相互作用モチーフ (hRpn10VWA) を欠く,切り離されたhRpn10を発現する細胞の分析.
  • プロテオミクおよびトランスクリプトミク分析により,調節不良のタンパク質と遺伝子発現パターンを特定します.
  • 観察されたフェノタイプにおけるOTUD5蓄積の役割を調査する.

主要な成果:

  • hRpn10のユビキチン結合活性を失うと,タンパク質の重要な失調が起こります.
  • 変異したタンパク質は,組織特異的要因の過剰表現を示し,細胞のアイデンティティの喪失を示します.
  • OTUD5の蓄積は,デウビキチナゼと転写レギュレータであり,転写不調に寄与する.

結論:

  • プロテアソームに依存するメカニズムは,細胞タイプ特異的な遺伝子発現プログラムを保護するために不可欠です.
  • プロテアソームの機能は,基本的な細胞維持を超えて,細胞のアイデンティティの統治を含みます.
  • hRpn10/PSMD4におけるユビキチン結合の障害は,転写不調によって細胞のアイデンティティを損なう.