血液サンプルを長期保存した結果,血小板機能検査の結果への影響:in vitroの安定性研究
Artur Hausch1, Maria Deisenhammer1, Nenad Mitrovic2
1Department of Laboratory Medicine, Hospital Voecklabruck, Austria.
Scandinavian journal of clinical and laboratory investigation
|February 14, 2026
まとめ
ほとんどの血小板機能検査では,血液サンプルを最大5時間保存することが許容されるが,PFA-200コラーゲン/EPI検査では,重大な偏差が認められた.
科学分野:
- 臨床化学 臨床化学について
- 血液学 ヘマトロジ
- 実験室医学は,研究室医学である.
背景:
- 血小板機能検査は,出血リスクを評価し,抗血小板治療の指針を定める上で極めて重要です.
- 標準プロトコルはしばしば血液サンプル保存時間を制限し,実験室のワークフローと患者のケアに影響を及ぼします.
- 長期保存条件下でのサンプル安定性の理解は,信頼性の高い診断結果のために不可欠です.
研究 の 目的:
- 拡張された室温貯蔵 (5時間まで) が様々な血小板機能検査に与える影響を評価する.
- メーカーが推奨する保存期間を超えて,血小板機能試験結果の安定性を決定する.
- どの特定の血小板機能検査が,サンプルを長期保存した後に信頼性があるかを特定する.
主な方法:
- 30人の患者の血液サンプルを採取後 (ベースライン) と室温で3~4時間,5時間保存した直後に検査した.
- 血小板機能は,PFA-200 (コラーゲン/EPI,コラーゲン/ADP),マルチプレート (ASPI,ADP,TRAP),およびVerifyNow (アスピリン,PRU) 分析器を使用して評価されました.
- 安定性は,ベースライン値からの相対偏差の中央値 (テストの種類によって<19%または<28%) によって定義されました.
主要な成果:
- ほとんどの試験 (コラーゲン/ADP,ASPI,ADP,TRAP,アスピリン,PRU) は,5時間の貯蔵後,定義された限界を下回る中位偏差で許容可能な安定性を示しました.
- PFA-200コラーゲン/EPIテストは,5時間の保存後,平均35%の大幅な偏差を示しました.
- コラーゲン/EPIテストの4時間と5時間の貯蔵の間に有意な差異は認められず,ベースライン測定の潜在的な問題を示唆しています.
結論:
- 血液サンプルは,いくつかの主要な血小板機能検査のために室温で最大5時間保存され,実験室の柔軟性を高めることができます.
- PFA-200 コラーゲン/EPI 試験結果は,試験用カートリッジの問題により,長期保存後に信頼性がない可能性があります.
- PFA-200コラーゲン/EPIアッセイで観察された不一致を明らかにするために,さらなる調査が必要である.
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