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関連する概念動画

RNA Interference01:23

RNA Interference

28.2K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.2K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

27.2K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
27.2K
RNA Stability01:53

RNA Stability

35.8K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.8K
Alternative RNA Splicing02:18

Alternative RNA Splicing

25.3K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
25.3K
RNA Splicing01:32

RNA Splicing

60.7K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.7K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

14.9K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
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関連する実験動画

Updated: Feb 16, 2026

Author Spotlight: A Computational Pipeline for Analyzing Chimeric Noncoding RNA-Target RNA Interactions in High-Throughput Sequencing Data
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Author Spotlight: A Computational Pipeline for Analyzing Chimeric Noncoding RNA-Target RNA Interactions in High-Throughput Sequencing Data

Published on: December 1, 2023

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標的型単細胞RNAシーケンシング技術に関する実用的なガイド

Giulia Moro1, Erich Brunner2, Konrad Basler2

  • 1Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland. giulia.moro2@uzh.ch.

Communications biology
|February 14, 2026
PubMed
まとめ
この要約は機械生成です。

単細胞RNAシーケンシング (scRNA-seq) のバイアスは,トランスクリプト検出を制限する. このレビューは,これらの制限を詳細に説明し,研究者のトランスクリプトと領域の識別を改善するためのターゲットを絞ったシーケンシングソリューションを紹介しています.

さらに関連する動画

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets
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Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

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Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
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Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

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関連する実験動画

Last Updated: Feb 16, 2026

Author Spotlight: A Computational Pipeline for Analyzing Chimeric Noncoding RNA-Target RNA Interactions in High-Throughput Sequencing Data
07:35

Author Spotlight: A Computational Pipeline for Analyzing Chimeric Noncoding RNA-Target RNA Interactions in High-Throughput Sequencing Data

Published on: December 1, 2023

1.2K
Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets
11:34

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

Published on: July 18, 2019

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Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
06:38

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Published on: October 12, 2018

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科学分野:

  • 分子生物学は分子生物学である.
  • ゲノミクスゲノミクスとは
  • バイオインフォマティックス

背景:

  • 現在の単細胞RNAシーケンシング (scRNA-seq) 方法は,細胞トランスクリプトの10-40%しか検出できません.
  • 既存の高通量 scRNA-seq 方法は,主に翻訳されていない領域を捕捉し,内部トランスクリプトの詳細を失います.

研究 の 目的:

  • トランスクリプトと領域検出を制限する scRNA-seq プロトコルのバイアスを概説する.
  • scRNA-seqデータを強化するための標的型シーケンシングソリューションをレビューする.
  • 適切なターゲットメソッドを選択するための意思決定ツリーを提供する.

主な方法:

  • scRNA-seqプロトコルバイアスのレビュー.
  • プロトコルステップに基づいてターゲティングされたシーケンシングソリューションの分類.
  • 方法選択のための意思決定の枠組みの開発.

主要な成果:

  • scRNA-seqプロトコルステップにおける主要なバイアスの特定.
  • ターゲティングシーケンシングのアプローチを5つのカテゴリーに分類する.
  • 実験設計の指針となる意思決定ツリーのプレゼンテーション.

結論:

  • ターゲティングシーケンシングは,scRNA-seqの制限を克服するための解決策を提供します.
  • プロトコルバイアスの理解は,最適な方法を選択するために重要です.
  • 意思決定ツリーは,scRNA-seq研究におけるトランスクリプトと領域検出の改善に研究者を助けます.