Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Ribosome Profiling02:24

Ribosome Profiling

4.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.2K
RNA-seq03:21

RNA-seq

12.2K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.2K
Protein Folding Quality Check in the RER01:29

Protein Folding Quality Check in the RER

5.3K
ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
5.3K
Riboswitches01:56

Riboswitches

9.8K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
9.8K
Genomics02:02

Genomics

40.9K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.9K
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

24.7K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
24.7K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

AI in Cancer Prognosis: A Systematic Review of Multimodal Models Combining Pathology Images and High-Throughput Omics.

Cancer informatics·2026
Same author

Expanding the human proteome with microproteins and peptideins.

Nature·2026
Same author

An expanded reference catalog of translated open reading frames for biomedical research.

Nucleic acids research·2026
Same author

Held out wings RNA binding activity in the cytoplasm during early spermatogenesis.

Communications biology·2026
Same author

The complete genome of a songbird.

bioRxiv : the preprint server for biology·2026
Same author

Translon: a single term for translated regions.

Nature methods·2025
Same journal

Region-aware bridge modeling enables interpretable mesoscale representation of spatial transcriptomic tissue sections.

Bioinformatics advances·2026
Same journal

Microbiome differential abundance methodologies to detect relevant taxa associated with chemotherapy toxicity rate in colorectal cancer.

Bioinformatics advances·2026
Same journal

maldipickr dereplicates microbial MALDI-TOF spectra to facilitate multiplexed isolation.

Bioinformatics advances·2026
Same journal

RAM-MSA: an anytime memory-bounded method for exact multiple sequence alignment using path finding.

Bioinformatics advances·2026
Same journal

Interpretable machine learning for low-sample multi-omics: a case study of ferret vaccine response.

Bioinformatics advances·2026
Same journal

DeepTaxa: a hybrid CNN-BERT framework for 16S rRNA taxonomic classification.

Bioinformatics advances·2026
関連記事をすべて見る

関連する実験動画

Updated: Feb 17, 2026

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

4.2K

InspectorORF: Ribo-Seqと追加のゲノムまたはトランスクリプトームデータを視覚化するためのツールです.

Eilidh L Ward1,2, Isabel Birds1,2, Mary J O'Connell3,4

  • 1School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom.

Bioinformatics advances
|February 16, 2026
PubMed
まとめ
この要約は機械生成です。

InspectorORFは,リボソームプロファイリングとRNAシーケンシングデータを可視化する新しいRパッケージです. このツールは,新しいオープン・リーディング・フレーム (ORF) を検証し,翻訳を理解するのに役立ちます.

さらに関連する動画

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
12:05

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

9.3K
A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

12.3K

関連する実験動画

Last Updated: Feb 17, 2026

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

4.2K
RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
12:05

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

9.3K
A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

12.3K

科学分野:

  • 分子生物学は分子生物学である.
  • ゲノミクスゲノミクスとは
  • バイオインフォマティックス

背景:

  • リボソームのプロファイリングは,トランスラトームの理解を進めており,多数の代替オープンリーディングフレーム (ORF) を明らかにしました.
  • 現在のORF検出ツールには,翻訳の検証と予測に不可欠な視覚検査機能が欠けている.
  • 新型ORFには,枠外,重複,上流,下流,および非正規のスタートコドン変種が含まれます.

研究 の 目的:

  • InspectorORFを提示するには,リボソームプロファイリングとRNAシーケンシングの可視化のためのRパッケージが読み込まれます.
  • 翻訳データの視覚的検査を可能にすることで,新しいORFの検証と予測を容易にする.
  • プロテオミクスとロングリードシーケンシングを含む多様な証拠を統合して,堅牢なORF検証を行う.

主な方法:

  • データ可視化のためのRパッケージであるInspectorORFの開発.
  • リボソームプロファイリングとRNAシーケンシングの統合は,比較分析のために読み取れます.
  • プロテオミックおよびロングリードシーケンシングデータを含むカスタマイズ可能なプロットオプション.

主要な成果:

  • InspectorORFは,転写信号と翻訳信号の区別を可能にします.
  • このパッケージは,統合されたデータ可視化を通じて,予測された新しいORFの検証を容易にする.
  • プロテオミクスとロングリードシーケンシングからの補足的な証拠は,強化された検証のために組み込まれることができます.

結論:

  • InspectorORFは,翻訳と新しいORF発見を研究する研究者のための貴重なツールを提供します.
  • リボソームプロファイリングデータの視覚検査は,正確なORF検証に不可欠です.
  • マルチオミックスのデータの統合分析により,新しいORF識別の信頼性が向上します.