ヤロウィア・リポリティカで,前駆体供給工学と給餌バッチ発酵最適化によるハイタイターデノボラベリーケトン生産
Yuanyuan Li1, Shuhao Niu1, Zixuan Wang1
1School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Synthetic Biology of Jiangsu Province, Jiangnan University, Wuxi 214122, China.
Bioresource technology
|February 17, 2026
まとめ
ラズベリーケトン (RK) の微生物産生は,前駆体供給を改善するために代謝経路を設計することによって,Yarrowia lipolyticaで強化されました. これにより,発酵による最高デノボRKタイターが報告されました.
科学分野:
- メタボリックエンジニアリング
- 合成生物学 合成生物学とは
- 産業微生物学 産業微生物学とは
背景:
- ラズベリーケトン (RK) は,貴重な味と香りの化合物です.
- RKのデノボ微生物合成は,前駆体 (l-チロシンとマロニル-CoA) の可用性によって制限されています.
研究 の 目的:
- ヤロウイア・リポリティカ (Yarrowia lipolytica) を,グルコースから高タイトルのラズベリーケトン生物合成 (de novo raspberry ketone biosynthesis) をするために設計する.
- 強化されたRK生産のための前駆者の制限を克服するために.
主な方法:
- 26S rDNAロカスに多複製RK経路を統合しました.
- シキマートとペントースリン酸経路を強化し,l-チロシンおよび関連する前駆体供給を増やす.
- ベータ酸化を強化し,マロニル-CoAの制限に対処するために代替マロニル-CoA経路を導入しました.
- 栄養バッチ発酵条件を最適化しました.
主要な成果:
- 初期株と比較してRKタイターの42.5倍の改善を達成しました.
- 5Lの給餌バッチ発酵で最終RK濃度7.24g/Lに達しました.
- 微生物発酵でこれまでに報告された最高de novo RKタイターを示した.
結論:
- メタボリックエンジニアリングの戦略は,Yarrowia lipolytica.でRK前駆体供給を成功裏に強化しました.
- 発酵条件の最適化により,RKの生産がさらに増加しました.
- この研究は,高チーターの微生物RKバイオシンセシスのための堅牢なプラットフォームを提示しています.
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