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Updated: Feb 20, 2026

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
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触媒pKa 遺伝コード拡張による水解性金属酵素の衰弱

Benjamin P Manser1, Alexandria Deliz Liang1

  • 1Department of Chemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.

Biochemistry
|February 18, 2026
PubMed
まとめ

遺伝子コードの拡張により,金属ヒドローラゼが酸性活性を増強するために設計されました. 重要なヒスティジン残基の変異により,低 pH レベルでの酵素耐性および触媒効率が向上しました.

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